Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 5: 391-401, 1959;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coppini, D.
Right arrow Articles by Montorsi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coppini, D.
Right arrow Articles by Montorsi, M.

Clinical Chemistry, Vol 5, 391-401, Copyright © 1959 by the American Association for Clinical Chemistry

Quantitative Determination of Tryptophan Metabolites (via Kynurenine) in Biologic Fluids

D. Coppini 1, C. A. Benassi 1, and M. Montorsi 1

1 Institutes of Pharmaceutical Chemistry of the Universities of Padova and Modena, Italy.

A simple and rapid method for the quantitative determination of eight tryptophan (via kynurenine) metabolites is described. The following compounds can be determined even when present in the same sample of urine, blood or spinal fluid: kynurenine, 3-hydroxykynurenine, Nalpha-acetylkynurenine, anthranilic and 3-hydroxyanthranilic acids, kynurenic and xanthurenic acids, 8-methyl ether of the xanthurenic acid.

The compounds are separated by means of two-dimensional chromatography. Spots of kynurenic acid, Nalpha-acetylkynurenine and xanthurenic acid 8-methyl ether are eluted with ethanol and the absorbancies are read in the ultraviolet.

An acetic solution of p-dimethylaminobenzaldehyde serves as eluent and chromogenic reagent for the spots of kynurenine and anthranilic acid.

Spots corresponding to 3-hydroxykynurenine, xanthurenic and 3-hydroxyanthranilic acids are eluted with water and determined by means of the color developed with a diazo reagent.

Kynurenic and xanthurenic acids can also be determined conveniently by means of a spectrophotofluorometric procedure.

The reproducibility of the results is satisfactory over a large range, and very good for amounts of 1 to 5 µg.

Submitted on February 3, 1959






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1959 by the American Association for Clinical Chemistry.