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Clinical Chemistry 5: 402-404, 1959;
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Clinical Chemistry, Vol 5, 402-404, Copyright © 1959 by the American Association for Clinical Chemistry

Variation of pH of Clinical Samples as a Source of Error in Enzyme Determinations

Richard J. Henry 1 and Neil Chiamori 1

1 Bio-Science Research Foundation, 12318 Santa Monica Blvd., Los Angeles, Calif.

Variation of pH of urine and serum samples, either physiologic or that resulting from change on standing, may be a significant source of error in determination of enzyme activity. Buffer systems employed to control pH may be inadequate to cope with the variations which may be encountered. It has been demonstrated that such is the case in the determination of serum alkaline phosphatase by the method of Shinowara, Jones and Reinhart.

Submitted on January 2, 1959






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Copyright © 1959 by the American Association for Clinical Chemistry.