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Clinical Chemistry 5: 446-451, 1959;
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Clinical Chemistry, Vol 5, 446-451, Copyright © 1959 by the American Association for Clinical Chemistry

A Discontinuous Buffer System for Paper Electrophoresis of Human Hemoglobins

C. A. J. Goldberg 1

1 William Pepper Laboratory of Clinical Medicine, University of Pennsylvania, Philadelphia, Pa.

A method for paper electrophoresis of hemoglobins in a discontinuous buffer system is presented. Tris-EDTA- borate buffer 0.12M., pH 9.1, is applied to the paper. Barbital buffer 0.06 M., pH 8.6, is used in the buffer vessels.

The discontinuous buffer system enables the detection of hemoglobin A2 in small samples of hemolysate. Resolution of abnormal hemoglobins is superior to that in barbital or Tris-EDTA-borate buffer alone. It offers special advantage for the examination of aged samples of hemoglobin.

Reasons for the behavior of the hemoglobins in this buffer system are discussed.

Submitted on March 21, 1959






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Copyright © 1959 by the American Association for Clinical Chemistry.