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Clinical Chemistry 5: 470-478, 1959;
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Clinical Chemistry, Vol 5, 470-478, Copyright © 1959 by the American Association for Clinical Chemistry

Studies on the Use of the van den Bergh Reagent for Determination of Serum Bilirubin

Samuel Meites 1 and Cecelia K. Hogg 1

1 Clinical Laboratory, Columbus Children's Hospital, and the Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio.

1. By increasing the concentration of hydrochloric acid, sulfanilic acid, and sodium nitrite in the van den Bergh reagent used for determining total serum bilirubin it is possible to increase the absorbance of azobilirubin and to accelerate its reaction time to less than 10 minutes. A modified reagent is proposed for the Malloy and Evelyn procedure.

2. The effects of temperature, urea, and protein on the coupling of serum bilirubin are discussed.

3. Hemolysis seriously affects the coupling of bilirubin. The decrease in azobilirubin production appears to vary directly with the amount of hemolysis. When the amount of hemolysis is constant, the decrease in azobilirubin production appears to vary inversely with the bilirubin concentration. The interfering effect of hemoglobin is unexplained.

Submitted on February 7, 1959




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
S. Meites
History of Clinical Chemistry in a Children's Hospital (1914-1964)
Clin. Chem., July 1, 2000; 46(7): 1009 - 1013.
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Copyright © 1959 by the American Association for Clinical Chemistry.