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Clinical Chemistry 5: 479-487, 1959;
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Clinical Chemistry, Vol 5, 479-487, Copyright © 1959 by the American Association for Clinical Chemistry

Elimination of Interfering Chromogens in the Zimmerman Reaction for Measuring 17-Ketosteroids

Arnold G. Ware 1, James A. Demetriou 1, Solomon Notrica 1, Ronald Searcy 1, Clifford Walberg 1, and Fred Cox 1

1 Laboratory Division of Los Angeles County General Hospital and the Departments of Biochemistry and Nutrition and Surgery, University of Southern California.

A simple extraction technic is described for separating interfering chromogens from the 17-KS complex formed when the Zimmerman reaction is applied to urinary extracts. The procedure is performed immediately after the Zimmerman reaction is completed. It allows quantitation of the steroid present in a filter photometer at one wave length. Results compare favorably with those obtained after dilution of the Zimmerman complex with 70% aqueous ethanol and application of Allen's correction.

Submitted on February 21, 1959






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Copyright © 1959 by the American Association for Clinical Chemistry.