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Clinical Chemistry 5: 509-518, 1959;
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Clinical Chemistry, Vol 5, 509-518, Copyright © 1959 by the American Association for Clinical Chemistry

Measurement of Isocitric Dehydrogenase Activity in Body Fluids

George N. Bowers Jr. 1

1 William Pepper Laboratory of Clinical Medicine and the Department of Medicine, University of Pennsylvania, Philadelphia 4, Pa.

1. Factors influencing the isocitric dehydrogenase (ICD) activity of serum were studied. Substrate inhibition by high concentrations of isocitrate was observed.

2. The temperature-activity curve of serum ICD was determined. The importance of controlling the temperature of the reaction was stressed.

3. Using this information, a method of measuring the ICD activity in 0.2 ml. of serum is described.

4. The range of ICD activity in 50 healthy males was 30 to 192 units with a mean of 90 units and a standard deviation of ± 34.3 units.

Submitted on June 12, 1959




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Copyright © 1959 by the American Association for Clinical Chemistry.