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Clinical Chemistry 5: 532-541, 1959;
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Clinical Chemistry, Vol 5, 532-541, Copyright © 1959 by the American Association for Clinical Chemistry

Spectrophotometric Behavior of Azobilirubin and Azotaurobilirubin

M. Jirsa 1 and V. Jirsová 1

1 Laboratory for Research in Pathophysiology of Hematopoesis and Hepatic Diseases, Medical Clinic, Charles University, and The Institute for Care of Mother and Child, Prague, Czechoslovakia.

The authors studied the influence of pH, alcohol, and albumin on the spectrophotometric behavior of azo dyes prepared from direct and indirect bilirubin by coupling with sulphanilic acid. Synthetically prepared taurobilirubin was used as a model for direct bilirubin. It was found that extinction and absorption maximum values depend on all 3 factors. Most important for practical purposes is the observed rise of azo dye extinction in serum and in albumin caused by alcohol. This means, if the Malloy and Evelyn method for the quantitative colometric estimation of direct bilirubin in serum is used, that part of the directly reacting taurobilirubin is estimated as indirect bilirubin. Revision of colorimetric methods for direct bilirubin estimation in serum is recommended.

Submitted on January 22, 1959






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Copyright © 1959 by the American Association for Clinical Chemistry.