| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Endocrinology and Metabolism |
1 Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.
2 Medizinische Klinik IV (WE28) and3
Institut für Klinische Chemie und Pathobiochimie, Freie Universität Berlin, Klinikum Benjamin Franklin, Berlin, Germany.
4 Department of Medicine, University Medical Center Nijmegen St. Radboud, Nijmegen, The Netherlands.
aAddress correspondence to this author at: Department of Medicine-541, University Medical Center St. Radboud, Geert Grooteplein 8, 6525 GA Nijmegen, The Netherlands. Fax 31-24-3541734; e-mail j.deinum{at}aig.umcn.nl.
Background: Measurement of plasma renin is important for the treatment of patients with congenital adrenal hyperplasia (CAH) and in the evaluation of patients with suspected hyperaldosteronism. Immunologic assays for plasma renin offer easier implementation and standardization than enzyme-kinetic assays for plasma renin activity, but their sensitivity and specificity have been questioned. We studied a renin immunochemiluminescence assay on an automated platform.
Methods: Renin was measured by an enzymatic assay, by IRMA, and by the new Nichols Advantage Specialty System immunochemiluminometric assay (ICMA), in plasmas from unselected individuals from our outpatient departments and in samples from patients with selected diagnoses.
Results: The detection limit in the ICMA was 0.1 mU/L. The recovery was >90%, and the imprecision (CV) was generally <9%. Mean (SD) concentrations measured by ICMA were 32 (21)% lower than those measured by IRMA. Renin concentrations as measured by ICMA were identical in serum and EDTA-, heparin-, and citrate-anticoagulated plasmas. Prolonged incubation of whole blood at room temperature before centrifugation did not affect renin concentrations. The central 95% interval for 80 healthy adults was 6–85.5 mU/L. Plasma renin as assessed by ICMA in patients with primary hyperaldosteronism was <0.2 mU/L.
Conclusions: The performance characteristics of the new renin ICMA allow its use for patients with CAH and for the diagnosis of mineralocorticoid hypertension. In view of the variability of renin concentrations, use for other forms of hypertension or physiologic research calls for the development of uniform sampling protocols.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  K. S. Myint, M. Watts, D. S Appleton, D. J Lomas, N. Jamieson, K. P Taylor, S. Coghill, and M. J Brown Primary hyperaldosteronism due to adrenal microadenoma: a curable cause of refractory hypertension Journal of Renin-Angiotensin-Aldosterone System, June 1, 2008; 9(2): 103 - 106. [Abstract] [PDF]
|
|
|
|
 |
|
 R. S. Vasan, S. Demissie, M. Kimura, L. A. Cupples, N. Rifai, C. White, T. J. Wang, J. P. Gardner, X. Cao, E. J. Benjamin, et al. Association of Leukocyte Telomere Length With Circulating Biomarkers of the Renin-Angiotensin-Aldosterone System: The Framingham Heart Study Circulation, March 4, 2008; 117(9): 1138 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J Brown Renin: friend or foe? Heart, September 1, 2007; 93(9): 1026 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Wang, P. Gona, M. G. Larson, D. Levy, E. J. Benjamin, G. H. Tofler, P. F. Jacques, J. B. Meigs, N. Rifai, J. Selhub, et al. Multiple Biomarkers and the Risk of Incident Hypertension Hypertension, March 1, 2007; 49(3): 432 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.H. J. Danser and J. Deinum Renin, Prorenin and the Putative (Pro)renin Receptor Hypertension, November 1, 2005; 46(5): 1069 - 1076. [Full Text] [PDF]
|
|
|
|
|
|
A. J. Danser and J. Deinum Spotlight on Renin: Renin, Prorenin and the Putative (Pro)renin Receptor Journal of Renin-Angiotensin-Aldosterone System, September 1, 2005; 6(3): 163 - 165. [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
