Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 50: 306-312, 2004. First published December 18, 2003; 10.1373/clinchem.2003.024539
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
clinchem.2003.024539v1
50/2/306    most recent
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Biel, S. S.
Right arrow Articles by Gelderblom, H. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Biel, S. S.
Right arrow Articles by Gelderblom, H. R.
Related Collections
Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 2004;50:306-312.)
© 2004 American Association for Clinical Chemistry, Inc.

Molecular Diagnostics and Genetics

Detection of Human Polyomaviruses in Urine from Bone Marrow Transplant Patients: Comparison of Electron Microscopy with PCR

Stefan S. Biel1,3,a, Andreas Nitsche2,3, Andreas Kurth3, Wolfgang Siegert2, Muhsin Özel3 and Hans R. Gelderblom3

1 Beiersdorf AG, Hamburg, Germany.
2 Med. Klinik II, Charite-Campus Charite Mitte, Berlin, Germany.
3 Robert Koch Institute, Berlin, Germany.

aAddress correspondence to this author at: Analytical Microscopy Department, Beiersdorf AG, Unnastrasse 48, D-20245 Hamburg, Germany. Fax 49-40-4909-3855; e-mail stefan.biel{at}beiersdorf.com.

Background: We studied electron microscopy (EM) as an appropriate test system for the detection of polyomavirus in urine samples from bone marrow transplant patients.

Methods: We evaluated direct EM, ultracentrifugation (UC) before EM, and solid-phase immuno-EM (SPIEM). The diagnostic accuracy of EM was studied by comparison with a real-time PCR assay on 531 clinical samples.

Results: The detection rate of EM was increased by UC and SPIEM. On 531 clinical urine samples, the diagnostic sensitivity of EM was 47% (70 of 149) with a specificity of 100%. We observed a linear relationship between viral genome concentration and the proportion of urine samples positive by EM, with a 50% probability for a positive EM result for urine samples with a polyomavirus concentration of 106 genome-equivalents (GE)/mL; the probability of a positive EM result was 0% for urine samples with <103 GE/mL and 100% for urine samples containing 109 GE/mL.

Conclusions: UC/EM is rapid and highly specific for polyomavirus in urine. Unlike real-time PCR, EM has low sensitivity and cannot quantify the viral load.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
A. Nitsche, M. Buttner, S. Wilhelm, G. Pauli, and H. Meyer
Real-Time PCR Detection of Parapoxvirus DNA,
Clin. Chem., February 1, 2006; 52(2): 316 - 319.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Association for Clinical Chemistry.