Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 50: 1165-1173, 2004; 10.1373/clinchem.2003.030114
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saussoy, P.
Right arrow Articles by Latinne, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saussoy, P.
Right arrow Articles by Latinne, D.
Related Collections
Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 2004;50:1165-1173.)
© 2004 American Association for Clinical Chemistry, Inc.

Molecular Diagnostics and Genetics

Differentiation of Acute Myeloid Leukemia from B- and T-Lineage Acute Lymphoid Leukemias by Real-Time Quantitative Reverse Transcription-PCR of Lineage Marker mRNAs

Pascale Saussoy1,a, Jean-Luc Vaerman1, Nicole Straetmans4, Véronique Deneys1, Guy Cornu2, Augustin Ferrant3 and Dominique Latinne1

Cliniques Universitaires Saint Luc,1 Service de Biologie Hématologique, 2 Service de Pédiatrie, and 3 Service d’Hématologie, Brussels, Belgium. 4 Hôpital de Jolimont, Service d’Hématologie, Haine-Saint-Paul, Belgium.

aAddress correspondence to this author at: Cliniques Universitaires Saint Luc (UCL), Service de Biologie Hématologique, Clos Chapelle-aux-Champs 30-UCL 30.52, 1200 Bruxelles, Belgique. Fax 32-2-762-5855; e-mail Pascale.Saussoy{at}sang.ucl.ac.be.

Background: Flow cytometry of lineage markers is useful in the classification of leukemias. Our aim was to assess whether the study of lineage genes at the RNA level would enable differentiation of acute myeloid leukemias (AMLs) from B-and T-lineage acute lymphoid leukemias (ALLs).

Methods: We measured mRNA of four lineage markers [CD19, CD79a, CD3e, and myeloperoxidase (MPO)] by reverse transcription followed by real-time quantitative (RTQ)-PCR. We investigated 72 acute leukemias (40 AMLs with 23–93% blast cells plus 27 B-lineage ALLs and 5 T-lineage ALLs) defined by morphologic criteria at diagnosis. RTQ-PCR analysis was performed on bone marrow without cell sorting. The expression of each gene was calculated as the difference in the threshold cycle [{Delta}CT; CT value of target gene minus CT value of housekeeping gene (Abelson)].

Results: Three patterns of expression were detected. In the first, CD19, CD79a, and MPO mRNAs were less abundant than CD3e. In the second pattern, MPO mRNA was more abundant than the other three mRNAs. In the third, CD19 or CD79a was more highly expressed than CD3e and MPO. The three patterns corresponded to T-ALL, AML, and B-ALL, respectively. The use of cutoffs to establish qualitatively the pattern of coexpression of the four lineage markers provided the same information as the comparison among the four {Delta}CT values. Prospective use of the scoring system correctly classified each of 13 additional cases (8 AML, 4 B-lineage ALL, and 1 T-lineage ALL).

Conclusion: Study of lineage markers at diagnosis by RTQ-PCR allows differentiation of AML from B-ALL or T-ALL without cell sorting, even when the bone marrow contains few blast cells.




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Pathol.Home page
Q. H. Meng, G. Yang, W. Yang, B. Jiang, L. Wu, and R. Wang
Protective Effect of Hydrogen Sulfide on Balloon Injury-Induced Neointima Hyperplasia in Rat Carotid Arteries
Am. J. Pathol., April 1, 2007; 170(4): 1406 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
B. Martin, A. Jofre, M. Garriga, M. Pla, and T. Aymerich
Rapid Quantitative Detection of Lactobacillus sakei in Meat and Fermented Sausages by Real-Time PCR
Appl. Envir. Microbiol., September 1, 2006; 72(9): 6040 - 6048.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Yang, K. Cao, L. Wu, and R. Wang
Cystathionine {gamma}-Lyase Overexpression Inhibits Cell Proliferation via a H2S-dependent Modulation of ERK1/2 Phosphorylation and p21Cip/WAK-1
J. Biol. Chem., November 19, 2004; 279(47): 49199 - 49205.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Association for Clinical Chemistry.