Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 51: 75-83, 2005; 10.1373/clinchem.2004.036467
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qin, Q.-P.
Right arrow Articles by Pettersson, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qin, Q.-P.
Right arrow Articles by Pettersson, K.
Related Collections
Right arrow Proteomics and Protein Markers
Right arrow Lipids, Lipoproteins, and Cardiovascular Risk Factors
(Clinical Chemistry. 2005;51:75-83.)
© 2005 American Association for Clinical Chemistry, Inc.

Proteomics and Protein Markers

Molecular Distinction of Circulating Pregnancy-Associated Plasma Protein A in Myocardial Infarction and Pregnancy

Qiu-Ping Qin1,a, Saara Kokkala1, Juha Lund2, Natalia Tamm3, Liisa-Maria Voipio-Pulkki4 and Kim Pettersson1

1 Department of Biotechnology, University of Turku, Turku, Finland.
2 Department of Medicine, University Central Hospital of Turku, Turku, Finland.
3 HyTest Ltd., Turku, Finland.
4 Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland.

aAddress correspondence to this author at: Department of Biotechnology, University of Turku, Tykistökatu 6A, Turku 20520, Finland. Fax 358-2-3338050; e-mail qiqin{at}utu.fi.

Background: In the blood of pregnant women, pregnancy-associated plasma protein A (PAPP-A) is present as a covalent complex with the proform of eosinophil major basic protein (proMBP). Recently, increased serum concentrations of PAPP-A have been found in acute coronary syndromes (ACS). The aim of this study was to investigate whether the circulating PAPP-A in ACS is the same as that in pregnancy.

Methods: We developed two time-resolved immunofluorometric assays based on a relative epitope map constructed by the use of 17 monoclonal antibodies. One assay, which measured total PAPP-A, used two PAPP-A subunit-specific antibodies. The other assay, which measured PAPP-A/proMBP complex, used one proMBP subunit-specific antibody and one PAPP-A subunit-specific antibody. Serum samples from four patients with myocardial infarction (MI), three pregnant women in their first trimester, and one in her third trimester were fractionated by gel filtration on a SuperoseTM 6 precision column. The two assays were used to analyze fractions obtained by gel filtration as well as serum samples serially collected from four other MI patients.

Results: Pregnancy-related PAPP-A was eluted as a single peak with a molecular mass of ~700 kDa, whereas ACS-related PAPP-A was also eluted as a single peak but with a molecular mass of ~530 kDa. Pregnancy-related PAPP-A was detected equally by the two assays, whereas increased ACS-related PAPP-A was detected only by the assay for total PAPP-A.

Conclusions: Our results provide the first evidence that circulating ACS-related PAPP-A is different from circulating pregnancy-related PAPP-A in that it is not complexed with proMBP. These findings provide a solid foundation for the design of immunoassays to accurately measure atherosclerosis-associated plasma protein A in the circulation.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
C. Gyrup, M. Christiansen, and C. Oxvig
Quantification of Proteolytically Active Pregnancy-Associated Plasma Protein-A with an Assay Based on Quenched Fluorescence
Clin. Chem., May 1, 2007; 53(5): 947 - 954.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Wittfooth, Q.-P. Qin, J. Lund, I. Tierala, K. Pulkki, H. Takalo, and K. Pettersson
Immunofluorometric Point-of-Care Assays for the Detection of Acute Coronary Syndrome-Related Noncomplexed Pregnancy-Associated Plasma Protein A
Clin. Chem., September 1, 2006; 52(9): 1794 - 1801.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Fredericks, V. Bertomeu-Gonzalez, I. Petrovic, D. W. Holt, and J. C. Kaski
Comment on Immunoassays Developed for Pregnancy-Associated Plasma Protein-A (PAPP-A) in Pregnancy May Not Recognize PAPP-A in Acute Coronary Syndromes.
Clin. Chem., August 1, 2006; 52(8): 1619 - 1620.
[Full Text] [PDF]

Home page
 Eur Heart JHome page
J. C. Kaski and D. W. Holt
Pregnancy-associated plasma protein-A and cardiovascular risk
Eur. Heart J., July 2, 2006; 27(14): 1637 - 1639.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Mueller, B. Dieplinger, W. Poelz, and M. Haltmayer
Increased Pregnancy-Associated Plasma Protein-A as a Marker for Peripheral Atherosclerosis: Results from the Linz Peripheral Arterial Disease Study
Clin. Chem., June 1, 2006; 52(6): 1096 - 1103.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
Q.-P. Qin, S. Kokkala, J. Lund, N. Tamm, X. Qin, M. Lepantalo, and K. Pettersson
Immunoassays Developed for Pregnancy-Associated Plasma Protein-A (PAPP-A) in Pregnancy May Not Recognize PAPP-A in Acute Coronary Syndromes
Clin. Chem., March 1, 2006; 52(3): 398 - 404.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the American Association for Clinical Chemistry.