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Clinical Chemistry 51: 1836-1844, 2005. First published August 17, 2005; 10.1373/clinchem.2005.052845
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(Clinical Chemistry. 2005;51:1836-1844.)
© 2005 American Association for Clinical Chemistry, Inc.

Automation and Analytical Techniques

Microfluidic Device for Rapid (<15 min) Automated Microarray Hybridization

Régis Peytavi1, Frédéric R. Raymond1, Dominic Gagné1, François J. Picard1, Guangyao Jia2, Jim Zoval2, Marc Madou2, Karel Boissinot1, Maurice Boissinot1, Luc Bissonnette1, Marc Ouellette1 and Michel G. Bergeron1,a

1 Centre de Recherche en Infectiologie de l’Université Laval, Centre Hospitalier Universitaire de Québec (Pavillon CHUL), Sainte-Foy, Québec, Canada.
2 Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA.

aAddress correspondence to this author at: Centre de Recherche en Infectiologie de l’Université Laval, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, 2705 Boul. Laurier, Sainte-Foy, Québec, Canada, G1V 4G2. Fax 418-654-2715; e-mail Michel.G.Bergeron{at}crchul.ulaval.ca.

Background: Current hybridization protocols on microarrays are slow and need skilled personnel. Microfluidics is an emerging science that enables the processing of minute volumes of liquids to perform chemical, biochemical, or enzymatic analyzes. The merging of microfluidics and microarray technologies constitutes an elegant solution that will automate and speed up microarray hybridization.

Methods: We developed a microfluidic flow cell consisting of a network of chambers and channels molded into a polydimethylsiloxane substrate. The substrate was aligned and reversibly bound to the microarray printed on a standard glass slide to form a functional microfluidic unit. The microfluidic units were placed on an engraved, disc-shaped support fixed on a rotational device. Centrifugal forces drove the sample and buffers directly onto the microarray surface.

Results: This microfluidic system increased the hybridization signal by ~10fold compared with a passive system that made use of 10 times more sample. By means of a 15–min automated hybridization process, performed at room temperature, we demonstrated the discrimination of 4 clinically relevant Staphylococcus species that differ by as little as a single-nucleotide polymorphism. This process included hybridization, washing, rinsing, and drying steps and did not require any purification of target nucleic acids. This platform was sensitive enough to detect 10 PCR-amplified bacterial genomes.

Conclusion: This removable microfluidic system for performing microarray hybridization on glass slides is promising for molecular diagnostics and gene profiling.




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