Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 51: 1955-1961, 2005. First published August 4, 2005; 10.1373/clinchem.2005.053348
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
clinchem.2005.053348v1
51/10/1955    most recent
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Honda, N.
Right arrow Articles by Takei, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Honda, N.
Right arrow Articles by Takei, H.
Related Collections
Right arrow Oak Ridge Conference
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 2005;51:1955-1961.)
© 2005 American Association for Clinical Chemistry, Inc.

Oak Ridge Conference

Simultaneous Multiple Immunoassays in a Compact Disc–Shaped Microfluidic Device Based on Centrifugal Force

Nobuo Honda1,a, Ulrika Lindberg2, Per Andersson2, Stephan Hoffmann2 and Hiroyuki Takei1

1 Fujirebio, Inc., Tokyo, Japan
2 Gyros AB, Uppsala, Sweden.

aAddress correspondence to this author at: Methodology Research Group, Research & Development Division, Fujirebio Inc., 51, Komiya-cho, Hachioji-shi, 192-0031, Tokyo, Japan. Fax 81-426-46-8325; e-mail no-honda{at}fujirebio.co.jp.


Abstract

Background: We explored the potential of a microfluidic device based on centrifugal force as an immunoassay platform by examining the imprecision of assays carried out with 200 nL of sample.

Methods: Biotinylated antibodies against {alpha}-fetoprotein (AFP), interleukin-6 (IL-6), and carcinoembryonic antigen [(CEA); 0.1 g/L each in 15 mmol/L phosphate-buffered saline (PBS) containing 0.1 mL/L Tween 20] were attached to a microcolumn packed with streptavidin-coated particles. A 200-nL sample was then allowed to pass through the microcolumn for 240 s, followed by Alexa 647–labeled detection antibody (7.5 mg/L in 15 mmol/L PBS containing 10 g/L bovine serum albumin). The flow rate was controlled by altering the rotational speed. Up to 104 sandwich type immunoassays were completed within 50 min.

Results: For AFP, IL-6, and CEA the detection limits were, respectively, 0.15, 1.25, and 1.31 pmol/L. Inter- and intraassay imprecisions (CVs) were <10% and <20%, respectively, for analyte concentrations >5 pmol/L. The CEA antibody had the lowest affinity according to fluorescence image analysis of the microcolumn region. The result was confirmed in a comparative study using BIAcore 3000.

Conclusions: Day-to-day (total) imprecision (CV) of immunoassays on the compact disc–shaped device are <20%. Analysis of fluorescence images allows rapid ranking of antibodies according to their affinities.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the American Association for Clinical Chemistry.