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Proteomics and Protein Markers |
Departments of1
Medical Biotechnology and 2
Biochemistry and Molecular Biology, Chang Gung University, Taoyuan, Taiwan.
3 Department of Radiation Oncology, 4
Division of Hematology/Oncology, Department of Internal Oncology, and 5
Department of Otorhinolaryngology, Section of Head and Neck Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
aAddress correspondence to this author at: Graduate School of Medical Biotechnology, Chang Gung University, 259 Wen-Hwa 1st Road, Taoyuan 333, Taiwan. Fax 886-3-2118247; e-mail ajchen{at}mail.cgu.edu.tw.
Background: There is no plasma marker for detecting oral cancer, one of the most frequent cancers worldwide. We developed a bead-based affinity-fractionated proteomic method to discover a novel plasma marker for oral cancer.
Methods: Affinity purification of heparinized plasma with magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential oral cancer markers. We compiled MS protein profiles for 57 patients with oral cancer and compared them with profiles from 29 healthy controls. The spectra were analyzed statistically using flexAnalysisTM and ClinProtTM bioinformatic software. In each MS analysis, the peak intensities of interest were normalized with an internal standard (adrenocorticotropic hormone 18–39). For identification, affinity bead–purified plasma protein was subjected to MALDI TOF/TOF analysis followed by Mascot identification of the peptide sequences and a search of the National Center for Biotechnology Information protein database.
Results: To optimize MALDI-TOF analysis based on the best discriminator of the cancer and control spectra, copper-chelated beads were used for plasma protein profiling. The within- and between-run CVs for assays were <4% and 7%, respectively. Six markers that differentiated between cancer and control spectra were found, with mean (SD) molecular masses of 2664 (1), 2850 (1), 3250 (1), 7735 (2), 7927 (2), and 9240 (2) Da. The 2664-Da marker, identified as a fragment of the fibrinogen 
-chain, had the highest sensitivity (100%) and specificity (97%) for cancer.
Conclusion: The high specificity and sensitivity of the fibrinogen -chain fragment suggest that it may be a clinical useful tumor marker.
The following articles in journals at HighWire Press have cited this article:
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  Y.-X. Qi, M.-J. Qu, D.-K. Long, B. Liu, Q.-P. Yao, S. Chien, and Z.-L. Jiang Rho-GDP dissociation inhibitor alpha downregulated by low shear stress promotes vascular smooth muscle cell migration and apoptosis: a proteomic analysis Cardiovasc Res, October 1, 2008; 80(1): 114 - 122. [Abstract] [Full Text] [PDF]
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 W. H. Moretz III, C. G. Gourin, D. J. Terris, Z.-S. Xia, Z. Liu, P. M. Weinberger, E. Chin, and B.-L. Adam Detection of Papillary Thyroid Carcinoma With Serum Protein Profile Analysis Arch Otolaryngol Head Neck Surg, February 1, 2008; 134(2): 198 - 202. [Abstract] [Full Text] [PDF]
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C. G. Gourin, W. H. Moretz III, P. M. Weinberger, Z.-S. Xia, Z. Liu, D. J. Terris, and B.-L. Adam Serum Protein Profile Analysis Following Definitive Treatment in Patients With Head and Neck Squamous Cell Carcinoma Arch Otolaryngol Head Neck Surg, November 1, 2007; 133(11): 1125 - 1130. [Abstract] [Full Text] [PDF]
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 R. K.T. Kam, T. C.W. Poon, H. L.Y. Chan, N. Wong, A. Y. Hui, and J. J.Y. Sung High-Throughput Quantitative Profiling of Serum N-Glycome by MALDI-TOF Mass Spectrometry and N-Glycomic Fingerprint of Liver Fibrosis Clin. Chem., July 1, 2007; 53(7): 1254 - 1263. [Abstract] [Full Text] [PDF]
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J. Albrethsen Reproducibility in Protein Profiling by MALDI-TOF Mass Spectrometry Clin. Chem., May 1, 2007; 53(5): 852 - 858. [Abstract] [Full Text] [PDF]
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W. C.S. Cho, T. T.C. Yip, R. K.C. Ngan, T.-T. Yip, V. N. Podust, C. Yip, H. H.Y. Yiu, V. Yip, W.-W. Cheng, V. W.S. Ma, et al. ProteinChip Array Profiling for Identification of Disease- and Chemotherapy-Associated Biomarkers of Nasopharyngeal Carcinoma Clin. Chem., February 1, 2007; 53(2): 241 - 250. [Abstract] [Full Text] [PDF]
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G. L. Hortin The MALDI-TOF Mass Spectrometric View of the Plasma Proteome and Peptidome Clin. Chem., July 1, 2006; 52(7): 1223 - 1237. [Abstract] [Full Text] [PDF]
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