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Clinical Chemistry 51: 966-972, 2005. First published April 15, 2005; 10.1373/clinchem.2004.042671
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(Clinical Chemistry. 2005;51:966-972.)
© 2005 American Association for Clinical Chemistry, Inc.

Proteomics and Protein Markers

Fast and Slow Skeletal Troponin I in Serum from Patients with Various Skeletal Muscle Disorders: A Pilot Study

Jeremy A. Simpson1, Ralf Labugger1, Christine Collier2, Robert J. Brison3, Steve Iscoe1,a and Jennifer E. Van Eyk1,2

Departments of1 Physiology, 2 Pathology, and 3 Emergency Medicine, Queen’s University, Kingston, Ontario, Canada.

aAddress correspondence to this author at: Department of Physiology, Queen’s University, Kingston, Ontario, Canada K7L 3N6. Fax 613-533-6880; e-mail iscoes{at}post.queensu.ca.

Background: Detection of skeletal muscle injury is hampered by a lack of commercially available assays for serum markers specific for skeletal muscle; serum concentrations of skeletal troponin I (sTnI) could meet this need. Moreover, because sTnI exists in 2 isoforms, slow (ssTnI) and fast (fsTnI), corresponding to slow- and fast-twitch muscles, respectively, it could provide insight into differential injury/recovery of specific fiber types. The purpose of this study was to investigate whether the 2 isoforms of sTnI and their modified forms are present in the blood of patients with various skeletal muscle disorders.

Methods: Serial serum samples were obtained from 25 patients with various skeletal muscle injuries. Serum proteins were separated by a modified sodium dodecyl sulfate–polyacrylamide gel electrophoresis protocol followed by Western blotting for sTnI with monoclonal antibodies specific to ssTnI and fsTnI.

Results: We observed (a) intact and, in some cases, degraded sTnI products; (b) evidence of posttranslational modifications in addition to proteolysis; and (c) differential detectability of both skeletal isoforms in the same patient.

Conclusions: It is possible to monitor both sTnI isoforms; this could lead to the development of new diagnostic assays for skeletal muscle damage.




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