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Clinical Chemistry 51: 1605-1611, 2005. First published July 7, 2005; 10.1373/clinchem.2005.050120
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(Clinical Chemistry. 2005;51:1605-1611.)
© 2005 American Association for Clinical Chemistry, Inc.

Molecular Diagnostics and Genetics

Immunoquantitative PCR for Prion Protein Detection in Sporadic Creutzfeldt–Jakob Disease

Stéphanie Gofflot1, Manuel Deprez2, Benaïssa el Moualij1, Awad Osman3, Jean-François Thonnart1, Olivier Hougrand2, Ernst Heinen1 and Willy Zorzi1,a

1 Centre de Recherche sur les Protéines Prions (CRPP) - Service d’Histologie Humaine, Université de Liège-CHU, Liège, Belgium.
2 Laboratoire de Neuropathologie, Centre Hospitalier Universitaire de Liège, Liège, Belgium.
3 Roboscreen GmbH, Leipzig, Germany.

aAddress correspondence to this author at: Centre de Recherche sur les Protéines Prions (CRPP), University of Liège, CHU B36 Tour de Pharmacie, Avenue de l’Hôpital 1, B-4000 Liège, Belgium. Fax 32-(0)4-3664321; e-mail willy.zorzi{at}ulg.ac.be.

Background: The most common human prion disorder is Creutzfeldt–Jakob disease (CJD); it includes sporadic, familial, iatrogenic, and variant subtypes. Diagnostic tests aim at detection with the highest specificity of very small deposits of abnormal prion protein (PrP).

Methods: We used immunoquantitative PCR (iqPCR) to detect proteinase K–resistant PrP (PrPRes) in tissue from the middle frontal gyrus of 7 patients with sporadic CJD and 7 non-CJD cases. We compared iqPCR with routine optimized ELISA, Western blotting, and immunohistochemical analyses.

Results: The 4 methods showed similar 100% sensitivity and specificity for the diagnosis of CJD. Along with high specificity, however, iqPCR had a threshold for PrPRes detection at least 10-fold lower than that of the classic ELISA.

Conclusions: iqPCR is a new method for PrPRes detection that combines 100% specificity with a detection threshold at least 10-fold lower than classic techniques. This method may improve the detection of minute PrPRes deposits in tissues and body fluids and thus be useful for diagnostic and sterilization applications.




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A. Rajkovic, B. E. Moualij, M. Uyttendaele, P. Brolet, W. Zorzi, E. Heinen, E. Foubert, and J. Debevere
Immunoquantitative Real-Time PCR for Detection and Quantification of Staphylococcus aureus Enterotoxin B in Foods
Appl. Envir. Microbiol., October 1, 2006; 72(10): 6593 - 6599.
[Abstract] [Full Text] [PDF]


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