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Clinical Chemistry 52: 220-226, 2006. First published November 23, 2005; 10.1373/clinchem.2005.054627
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Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2006;52:220-226.)
© 2006 American Association for Clinical Chemistry, Inc.

Proteomics and Protein Markers

Western Blotting of Total Lysate of Helicobacter pylori in Cases of Atrophic Body Gastritis

Roberta Mini1,2, Bruno Annibale3,2, Edith Lahner3, Giulia Bernardini1, Natale Figura2 and Annalisa Santucci1,a

1 Dipartimento di Biologia Molecolare and 2 Dipartimento di Medicina Interna, Scienze Endocrino-Metaboliche e Biochimica, Policlinico "Le Scotte", Università degli Studi di Siena, Siena, Italy.
3 Digestive and Liver Disease Unit, University "La Sapienza", Dipartimento di Scienze Cliniche, Ospedale Sant’Andrea, Rome, Italy.

aAddress correspondence to this author at: Università degli Studi di Siena, Dipartimento di Biologia Molecolare, via Fiorentina 1, 53100 Siena, Italy. Fax 39-0577-234903; e-mail santucci{at}unisi.it.

Background: Atrophic body gastritis is considered the first important step in the histogenesis of gastric carcinoma, a multistep process starting from chronic gastritis and progressing through chronic atrophic gastritis, intestinal metaplasia, and dysplasia. Helicobacter pylori is involved in the induction of atrophic body gastritis, but documentation of H. pylori infection is difficult because of the progressive disappearance of the bacterium. Our study aimed to detect past H. pylori infection in patients with atrophic body gastritis.

Methods: We used Western blot analyses of whole bacterial protein lysate of 2 different strains to probe sera from 143 patients. All sera were analyzed by ELISA (Bio-Rad), and results of gastric histology were available for all patients.

Results: Among 111 patient sera previously classified as negative for H. pylori infection by ELISA, 106 (95.5%) were positive when assayed by immunoblotting.

Conclusions: Commercial diagnostic reagent sets may fail to detect H. pylori infection. Western blotting of whole bacterial protein extracts could provide the basis of a noninvasive serology tool able to assess previous infection with H. pylori in patients with atrophic body gastritis.




The following articles in journals at HighWire Press have cited this article:


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L. Veijola, A. Oksanen, P. Sipponen, and H. Rautelin
Evaluation of a Commercial Immunoblot, Helicoblot 2.1, for Diagnosis of Helicobacter pylori Infection
Clin. Vaccine Immunol., November 1, 2008; 15(11): 1705 - 1710.
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E Lahner, M Milione, G Delle Fave, B Annibale, and M Raderer
H pylori negative MALT lymphoma patients successfully treated with antibiotics: doubts about their H pylori negativity * Author's reply.
Gut, November 1, 2006; 55(11): 1669 - 1669.
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