| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Proteomics and Protein Markers |
1 Department of Medicine;2
the Research Center of Heart, Brain, Hormone, and Healthy Aging;3
Department of Biochemistry; and4
Genome Research Center, University of Hong Kong, Hong Kong, China.
5 Department of Laboratory Medicine, Sternberk Hospital, Sternberk, The Czech Republic.
6 Clinical Biochemistry Unit, Queen Mary Hospital, Hong Kong, China.
aAddress correspondence to this author at: Department of Medicine, University of Hong Kong, L8-43, 21 Sassoon Road, Hong Kong, China. Fax 852-28162095; e-mail amxu{at}hkucc.hku.hk.
Background: Adipocyte fatty acid–binding protein (A-FABP) is traditionally thought to be a cytosolic fatty acid chaperone expressed in adipocytes. Mice with targeted disruption of the A-FABP gene exhibit a striking phenotype with strong protection from insulin resistance, hyperglycemia, and atherosclerosis. The clinical relevance of these findings remains to be confirmed.
Methods: We used tandem mass spectrometry–based proteomic analysis to identify proteins secreted from adipocytes and present in human serum. We measured serum A-FABP concentrations in 229 persons (121 men and 108 women; age range, 33–72 years), including 100 lean [body mass index (BMI) <25 kg/m2] and 129 overweight/obese individuals (BMI >25 kg/m2) selected from a previous cross-sectional study.
Results: A-FABP was released from adipocytes and was abundantly present in human serum. Mean (SD) circulating concentrations of A-FABP were significantly higher in overweight/obese than in lean persons [32.3 (14.8) vs 20.0 (9.8) µg/L; P <0.001]. Age- and sex-adjusted serum A-FABP concentrations correlated positively (P <0.005) with waist circumference, blood pressure, dyslipidemia, fasting insulin, and the homeostasis model assessment insulin resistance index. Moreover, we observed a significant increase in A-FABP concentrations corresponding with increases in the number of components of the metabolic syndrome (P <0.05).
Conclusions: A-FABP is a circulating biomarker closely associated with obesity and components of the metabolic syndrome, and measurement of serum concentrations of A-FABP might be useful for clinical diagnosis of obesity-related metabolic and cardiovascular disorders.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  H. Shi, Q. Wang, Q. Zhang, L. Leng, and H. Li Tissue expression characterization of chicken adipocyte fatty acid-binding protein and its expression difference between fat and lean birds in abdominal fat tissue Poult. Sci., February 1, 2010; 89(2): 197 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Lamounier-Zepter, C. Look, J. Alvarez, T. Christ, U. Ravens, W.-H. Schunck, M. Ehrhart-Bornstein, S. R. Bornstein, and I. Morano Adipocyte Fatty Acid-Binding Protein Suppresses Cardiomyocyte Contraction: A New Link Between Obesity and Heart Disease Circ. Res., August 14, 2009; 105(4): 326 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Y. Yeung, K. S. L. Lam, Y. Wang, A. W. K. Tso, and A. Xu Serum Zinc-{alpha}2-Glycoprotein Correlates with Adiposity, Triglycerides, and the Key Components of the Metabolic Syndrome in Chinese Subjects J. Clin. Endocrinol. Metab., July 1, 2009; 94(7): 2531 - 2536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C-L. Lam, A. Xu, K. S-L. Lam, B. Lam, J. C-M. Lam, M. M-S. Lui, and M. S-M. Ip Serum adipocyte-fatty acid binding protein level is elevated in severe OSA and correlates with insulin resistance Eur. Respir. J., February 1, 2009; 33(2): 346 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E J Rhee, W Y Lee, C Y Park, K W Oh, B J Kim, K C Sung, and B S Kim The association of serum adipocyte fatty acid-binding protein with coronary artery disease in Korean adults Eur. J. Endocrinol., February 1, 2009; 160(2): 165 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kralisch, H. Stepan, J. Kratzsch, M. Verlohren, H.-J. Verlohren, K. Drynda, U. Lossner, M. Bluher, M. Stumvoll, and M. Fasshauer Serum levels of adipocyte fatty acid binding protein are increased in gestational diabetes mellitus Eur. J. Endocrinol., January 1, 2009; 160(1): 33 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C.Y. Yeung, A. Xu, A. W.K. Tso, W.S. Chow, N. M.S. Wat, C. H.Y. Fong, S. Tam, P. C. Sham, and K. S.L. Lam Circulating Levels of Adipocyte and Epidermal Fatty Acid-Binding Proteins in Relation to Nephropathy Staging and Macrovascular Complications in Type 2 Diabetic Patients Diabetes Care, January 1, 2009; 32(1): 132 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C.Y. Yeung, Y. Wang, A. Xu, S. C.W. Cheung, N. M.S. Wat, D. Y.T. Fong, C. H.Y. Fong, M.T. Chau, P. C. Sham, and K. S.L. Lam Epidermal fatty-acid-binding protein: a new circulating biomarker associated with cardio-metabolic risk factors and carotid atherosclerosis Eur. Heart J., September 1, 2008; 29(17): 2156 - 2163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cabre, I. Lazaro, J. Girona, J. M. Manzanares, F. Marimon, N. Plana, M. Heras, and L. Masana Plasma fatty acid binding protein 4 is associated with atherogenic dyslipidemia in diabetes J. Lipid Res., August 1, 2008; 49(8): 1746 - 1751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, D. C.Y. Yeung, M. Karpisek, D. Stejskal, Z.-G. Zhou, F. Liu, R. L.C. Wong, W.-S. Chow, A. W.K. Tso, K. S.L. Lam, et al. Serum FGF21 Levels Are Increased in Obesity and Are Independently Associated With the Metabolic Syndrome in Humans Diabetes, May 1, 2008; 57(5): 1246 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cabre, I. Lazaro, J. Girona, J. M. Manzanares, F. Marimon, N. Plana, M. Heras, and L. Masana Plasma Fatty Acid-Binding Protein 4 Increases with Renal Dysfunction in Type 2 Diabetic Patients without Microalbuminuria Clin. Chem., January 1, 2008; 54(1): 181 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W.K. Tso, A. Xu, P. C. Sham, N. M.S. Wat, Y. Wang, C. H.Y. Fong, B. M.Y. Cheung, E. D. Janus, and K. S.L. Lam Serum Adipocyte Fatty Acid Binding Protein as a New Biomarker Predicting the Development of Type 2 Diabetes: A 10-year prospective study in a Chinese cohort Diabetes Care, October 1, 2007; 30(10): 2667 - 2672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Gregor and G. S. Hotamisligil Thematic review series: Adipocyte Biology. Adipocyte stress: the endoplasmic reticulum and metabolic disease J. Lipid Res., September 1, 2007; 48(9): 1905 - 1914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mohlig, M. O Weickert, E. Ghadamgadai, A. Machlitt, B. Pfuller, A. M Arafat, A. F H Pfeiffer, and C. Schofl Adipocyte fatty acid-binding protein is associated with markers of obesity, but is an unlikely link between obesity, insulin resistance, and hyperandrogenism in polycystic ovary syndrome women Eur. J. Endocrinol., August 1, 2007; 157(2): 195 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.C.Y. Yeung, A. Xu, C.W.S. Cheung, N.M.S. Wat, M.H. Yau, C.H.Y. Fong, M.T. Chau, and K.S.L. Lam Serum Adipocyte Fatty Acid-Binding Protein Levels Were Independently Associated With Carotid Atherosclerosis Arterioscler Thromb Vasc Biol, August 1, 2007; 27(8): 1796 - 1802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Alvarez-Llamas, E. Szalowska, M. P. de Vries, D. Weening, K. Landman, A. Hoek, B. H. R. Wolffenbuttel, H. Roelofsen, and R. J. Vonk Characterization of the Human Visceral Adipose Tissue Secretome Mol. Cell. Proteomics, April 1, 2007; 6(4): 589 - 600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Xu, A. W.K. Tso, B. M.Y. Cheung, Y. Wang, N. M.S. Wat, C. H.Y. Fong, D. C.Y. Yeung, E. D. Janus, P. C. Sham, and K. S.L. Lam Circulating Adipocyte-Fatty Acid Binding Protein Levels Predict the Development of the Metabolic Syndrome: A 5-Year Prospective Study Circulation, March 27, 2007; 115(12): 1537 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, K. S. L. Lam, E. W. Kraegen, G. Sweeney, J. Zhang, A. W.K. Tso, W.-S. Chow, N. M.S. Wat, J. Y. Xu, R. L.C. Hoo, et al. Lipocalin-2 Is an Inflammatory Marker Closely Associated with Obesity, Insulin Resistance, and Hyperglycemia in Humans Clin. Chem., January 1, 2007; 53(1): 34 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bronsky, M. Karpisek, E. Bronska, M. Pechova, B. Jancikova, H. Kotolova, D. Stejskal, R. Prusa, and J. Nevoral Adiponectin, Adipocyte Fatty Acid Binding Protein, and Epidermal Fatty Acid Binding Protein: Proteins Newly Identified in Human Breast Milk Clin. Chem., September 1, 2006; 52(9): 1763 - 1770. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
