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Clinical Chemistry 52: 511-514, 2006; 10.1373/clinchem.2005.058537
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(Clinical Chemistry. 2006;52:511-514.)
© 2006 American Association for Clinical Chemistry, Inc.

Technical Briefs

Polymorphisms in the Interleukin-1 Gene Cluster in Children and Young Adults with Systemic Meningococcemia

Georg Endler3,1, Rodrig Marculescu3,1, Philipp Starkl3, Alexander Binder1, Gotho Geishofer1, Martin Müller1, Bettina Zöhrer1, Bernhard Resch2, Werner Zenz1,2, Christine Mannhalter3,2,a for the Central European Meningococcal Genetic Study Group

(Departments of1 General Pediatrics and2 Neonatology, Medical University of Graz, Graz, Austria;3 Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University Vienna, Vienna, Austria;

aaddress correspondence to this author at: Clinical Institute of Medical and Chemical Laboratory Diagnostic, Medical University Vienna, Vienna, Austria, Waehringer Guertel 18-20, 1090 Wien, Austria; fax 43-1-40400-2097, e-mail christine.mannhalter{at}meduniwien.ac.at)


Abstract

Background: An association has been described between mortality in children with meningococcal disease and functional polymorphisms in the interleukin-1 (IL1) cluster. We undertook a multicenter study to evaluate associations of these polymorphisms in a Central European population.

Patients and Methods: The study involved 95 Middle European pediatric hospitals. We collected blood samples from, and clinical information about, 285 previously healthy children with meningococcal infection. We used a newly developed multiplexed mutagenic separated PCR assay to analyze 6 polymorphisms within the IL1 cluster: IL1A (–889)C/T, IL1A (+4845)G/T, IL1B (–511)C/T, IL1B (–31)C/T, IL1B (+3954), and IL1RA (+2018)C/T. We studied the same polymorphisms in a comparison group of 481 healthy newborns.

Results: Genotype frequencies between patients and the comparison group differed significantly only for the IL1RA (+2018)C/T variant: The CC genotype was more frequent in patients (11%) than in healthy controls (5%; P = 0.008). In the patient group, the C allele was significantly more prevalent (67%) in nonsurvivors than in survivors (42%; P = 0.02).

Conclusion: The IL1RA (+2018)C/T polymorphism is associated with the risk of meningococcal disease and with its outcome.






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