Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 52: 671-679, 2006. First published February 23, 2006; 10.1373/clinchem.2005.060715
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplements
Right arrow All Versions of this Article:
clinchem.2005.060715v1
52/4/671    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Plymoth, A.
Right arrow Articles by Hancock, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Plymoth, A.
Right arrow Articles by Hancock, W. S.
Related Collections
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2006;52:671-679.)
© 2006 American Association for Clinical Chemistry, Inc.

Proteomics and Protein Markers

Rapid Proteome Analysis of Bronchoalveolar Lavage Samples of Lifelong Smokers and Never-Smokers by Micro-Scale Liquid Chromatography and Mass Spectrometry

Amelie Plymoth1,2,3, Ziping Yang1, Claes-Göran Löfdahl2, Ann Ekberg-Jansson4, Magnus Dahlbäck3, Thomas E. Fehniger3, György Marko-Varga3,a and William S. Hancock1

1 Barnett Institute, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA.
2 Department of Respiratory Medicine and Allergology, University Hospital of Lund, Lund, Sweden.
3 AstraZeneca, Department of Biological Sciences, Lund, Sweden.
4 Department of Respiratory Medicine and Allergology, Sahlgrenska University Hospital, Gothenburg, Sweden.

aAddress correspondence to this author at: AstraZeneca Research and Development, Scheelev. 8, Lund 22 187, Sweden. Fax 46-33-71-44; e-mail gyorgy.marko-varga{at}astrazeneca.com.

Background: The aim of this study was to determine whether relative qualitative and quantitative differences in protein expression could be related to smoke exposure or smoke-induced airway inflammation. We therefore explored and characterized the protein components found in bronchoalveolar lavage (BAL) fluid sampled from either lifelong smokers or never-smokers.

Methods: BAL fluid samples obtained by bronchoscopy from 60-year-old healthy never-smokers (n = 18) and asymptomatic smokers (n = 30) were analyzed in either pooled or individual form. Initial global proteomic analysis used shotgun digestion approaches on unfractionated BAL fluid samples (after minimal sample preparation) and separation of peptides by gradient (90-min) liquid chromatography (LC) coupled with on-line linear ion trap quadropole mass spectrometry (LTQ MS) for identification and analysis.

Results: LTQ MS identified 481 high- to low-abundance proteins. Relative differences in patterns of BAL fluid proteins in smokers compared with never-smokers were observed in pooled and individual samples as well as by 2-dimensional gel analysis. Gene ontology categorization of all annotated proteins showed a wide spectrum of molecular functions and biological processes.

Conclusions: The described method provides comprehensive qualitative proteomic analysis of BAL fluid protein expression from never-smokers and from smokers at risk of developing chronic obstructive pulmonary disease. Many of the proteins identified had not been detected in previous studies of BAL fluid; thus, the use of LC-tandem MS with LTQ may provide new information regarding potentially important patterns of protein expression associated with lifelong smoking.




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. J. Fischer, K. L. Goss, T. E. Scheetz, C. L. Wohlford-Lenane, J. M. Snyder, and P. B. McCray Jr.
Differential Gene Expression in Human Conducting Airway Surface Epithelia and Submucosal Glands
Am. J. Respir. Cell Mol. Biol., February 1, 2009; 40(2): 189 - 199.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S.-J. Li, M. Peng, H. Li, B.-S. Liu, C. Wang, J.-R. Wu, Y.-X. Li, and R. Zeng
Sys-BodyFluid: a systematical database for human body fluid proteome research
Nucleic Acids Res., January 1, 2009; 37(suppl_1): D907 - D912.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. L. Ventura, R. Higdon, L. Hohmann, D. Martin, E. Kolker, H. D. Liggitt, S. J. Skerrett, and C. E. Rubens
Staphylococcus aureus Elicits Marked Alterations in the Airway Proteome during Early Pneumonia
Infect. Immun., December 1, 2008; 76(12): 5862 - 5872.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Plymoth, C.-G. Lofdahl, A. Ekberg-Jansson, M. Dahlback, P. Broberg, M. Foster, T. E. Fehniger, and G. Marko-Varga
Protein Expression Patterns Associated with Progression of Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage of Smokers
Clin. Chem., April 1, 2007; 53(4): 636 - 644.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
E. S. Chen and D. R. Moller
Expression Profiling in Granulomatous Lung Disease
Proceedings of the ATS, January 1, 2007; 4(1): 101 - 107.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Association for Clinical Chemistry.