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Clinical Chemistry 52: 749-752, 2006. First published February 9, 2006; 10.1373/clinchem.2005.063628
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(Clinical Chemistry. 2006;52:749-752.)
© 2006 American Association for Clinical Chemistry, Inc.

Technical Briefs

Total Polyphenol Intake Estimated by a Modified Folin–Ciocalteu Assay of Urine

Elena Roura1, Cristina Andrés-Lacueva1, Ramon Estruch2 and Rosa M. Lamuela-Raventós1,a

[Departments of1 Nutrition and Food Science-CeRTA, and2 Internal Medicine, Hospital Clínic, Institut d’Investigació Biomèdica August Pi i Sunyer (IDIBAPS), University of Barcelona, Barcelona, Spain;

aaddress correspondence to this author at: Department of Nutrition and Food Science, University of Barcelona, Av/Joan XXIII s/n. 08028, Barcelona, Spain; fax 34-93-403-59-31, e-mail lamuela{at}ub.edu]


Abstract

Background: Plant polyphenols have been studied largely because of the possibility that they might underlie the protective effects afforded by fruit and vegetable intake against cancer and other chronic diseases. Measurement of polyphenol content excreted in urine as an indicator of polyphenol consumption may offer a routine screening method that could be used for these pathologies.

Methods: Thirty-six healthy volunteers each received 2 interventions, one with a polyphenol-rich food (cocoa beverage) and one with a polyphenol-free food (milk) as a control, in a randomized cross-over design with 1-week intervals. The total polyphenol content excreted in urine during the 6 h after consumption of the test meals was measured by a modified Folin-Ciocalteu assay after sample cleanup by solid-phase extraction.

Results: The mean (SD) concentrations of polyphenols excreted in the urine 6 h after consumption of the test meals differed significantly: 140.95 (49.27) mg catechin/g of creatinine after the polyphenol-rich meal vs 90.43 (46.07) mg catechin/g of creatinine after the control meal (P <0.05).

Conclusions: This method allows analysis of a large number of samples per day, which is ideal for use in epidemiologic studies and may enable estimation of polyphenol consumption and determination of their possible role in preventing of certain pathologies, such as cancer, cardiovascular and degenerative diseases.






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