Clinical Chemistry
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Clinical Chemistry 52: 886-888, 2006. First published March 23, 2006; 10.1373/clinchem.2005.053983
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(Clinical Chemistry. 2006;52:886-888.)
© 2006 American Association for Clinical Chemistry, Inc.

Technical Briefs

Improved Specificity of a New Homogeneous Assay for LDL-Cholesterol in Serum with Abnormal Lipoproteins

Yasumasa Iwasakia, Hiroyuki Matsuyama and Nobuo Nakashima

1 (Department of Clinical Laboratory, Nagoya University Hospital, Nagoya, Japan;

aaddress correspondence to this author at: Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kohasu, Oko-cho, Nankoku 783-8505, Japan; fax 81-88-880-2344, e-mail iwasaki{at}med.kochi-u.ac.jp)


Abstract

Background: Although a homogeneous assay for serum LDL-cholesterol (LDL-C) has become a routine clinical procedure, problems remain in assay performance characteristics.

Methods: We examined the performance of a recently developed automated homogeneous assay (New-Daiichi assay) for serum LDL-C and compared the results with those obtained by the current homogeneous method (Denka-Seiken assay) or by ultracentrifugation as a control.

Results: The New-Daiichi assay showed satisfactory basic performance characteristics such as reproducibility, linearity, and stability. There was no interference in the assay by various substances examined. The LDL-C values obtained with this method correlated well with those obtained by ultracentrifugation. In samples from patients with obstructive jaundice, both methods detected cholesterol from abnormal lipoproteins (such as lipoprotein-X and -Y), but the New-Daiichi assay was less reactive and more specific for LDL-C.

Conclusion: The new method has improved performance for the accurate measurement of LDL-C in clinical practice.




The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
C. Garcia-Hejl, P. Vest, C. Renard, A. Merens-Gonthier, A. Boukhira, and H. Thefenne-Astier
Falsely Low LDL Cholesterol Results and Cholestasis.
Clin. Chem., November 1, 2006; 52(11): 2125 - 2127.
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