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Clinical Chemistry 52: 1749-1755, 2006. First published July 20, 2006; 10.1373/clinchem.2006.068502
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Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 2006;52:1749-1755.)
© 2006 American Association for Clinical Chemistry, Inc.

Automation and Analytical Techniques

Automated Chemiluminescence-Immunoassay for Aldosterone during Dynamic Testing: Comparison to Radioimmunoassays with and without Extraction Steps

Caroline Schirpenbach1, Lysann Seiler2,3, Christiane Maser-Gluth4, Felix Beuschlein3, Martin Reincke1,a and Martin Bidlingmaier1

1 Ludwig-Maximilians-University, Medizinische Klinik Innenstadt, Munich, Germany.
2 Centre Hospitalier Universitaire Vaudois, Département de médecine interne Lausanne, Lausanne, Switzerland.
3 Albert-Ludwigs-University, Medical Department 2, Freiburg, Germany.
4 Ruprecht-Karls-University, Institute of Pharmacology, Heidelberg, Germany.

aAddress correspondence to this author at: Medizinische Klinik Innenstadt, Klinikum der LMU Mänchen, Ziemssenstr. 1, D-80336 Mänchen, Germany. Fax 49-89-5160-4428; E-mail martin.reincke{at}med.uni-muenchen.de.

Background: Measurements of aldosterone have become more common since the recognition that primary aldosteronism is a more frequent cause of hypertension than previously believed. Our aim was to compare concentrations reported by 4 assays for samples obtained after saline infusion during dynamic testing.

Methods: We tested 104 participants (27 with primary aldosteronism, 30 with essential hypertension, and 47 healthy controls) with the intravenous saline infusion test (2.0 L isotonic saline over 4 h), with repetitive sampling. In all blood samples, aldosterone concentration was measured by an in-house RIA after extraction and chromatography, by 2 commercially available RIAs without extraction (Aldosterone Maia, Adaltis; Active Aldosterone, Diagnostics Systems Laboratories) and by an automated CLIA (Advantage, Nichols Institute Diagnostics).

Results: Correlation coefficients for results of pairs of assays ranged from 0.74 to 0.98. Agreement between commercial assays and in-house RIA was best at the low to intermediate concentrations after saline infusion. Mean (SD) Adaltis and DSL RIA results were 2- to 3-times higher [healthy participants: 78 (25) ng/L and 56 (18) ng/L, respectively] than those obtained by Nichols CLIA [17 (8) ng/L] and in-house RIA [23 (18) ng/L]. Aldosterone concentrations measured by the Nichols CLIA were below the limit of detection (limit of the blank) in 27 of 47 healthy participants.

Conclusions: Aldosterone concentrations reported by the Adaltis and DSL nonextraction RIAs were consistently higher than those produced by the Nichols CLIA and the in-house RIA. The convenient Nichols CLIA showed better agreement with the in-house RIA, but the concentrations in healthy participants were frequently undetectable by this method. Uncritical application of cutoff values from the literature must be avoided.




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