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Clinical Chemistry 53: 1858-1860, 2007. First published August 3, 2007; 10.1373/clinchem.2006.076380
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(Clinical Chemistry. 2007;53:1858-1860.)
© 2007 American Association for Clinical Chemistry, Inc.

Technical Briefs

Stability of Soluble Adhesion Molecules, Selectins, and C-Reactive Protein at Various Temperatures: Implications for Epidemiological and Large-Scale Clinical Studies

Janine Hartweg1,2,a, Michael Gunter1, Rafael Perera2, Andrew Farmer1,2, Carole Cull1,1, Casper Schalkwijk3,2, Astrid Kok3, Harry Twaalfhoven3, Rury Holman1 and Andrew Neil1,2

1 Diabetes Trials Unit, Oxford Centre for Diabetes, Endocrinology and Medicine, University of Oxford, United Kingdom; 2 Division of Public Health and Primary Health Care, University of Oxford, United Kingdom; 3 Clinical Chemistry, Institute for Cardiovascular Research VU University Medical Centre, Amsterdam, The Netherlands

aaddress correspondence to this author at: Diabetes Trials Unit, Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, Oxford, OX3 7LJ, United Kingdom; fax 44 (0)1865 857240, e-mail janine.hartweg{at}ndm.ox.ac.uk


Abstract

Background: We assessed the impact of sample storage conditions on soluble vascular cell adhesion molecules (sVCAM), soluble intracellular adhesion molecules (sICAM-1), soluble (s)E-selectin, C-reactive protein (CRP), and sP-selectin.

Methods: Markers were measured by ELISA in venous blood from 10 healthy volunteers on aliquots stored as plasma or whole blood at 4, 21, or 30 °C for 1–5 days and after 1–5 freeze-thaw cycles. We compared results on these samples to results for samples processed immediately and stored at –80 °C. Statistical models assessed time-related effects and effects of postprocessing conditions.

Results: Using an upper limit of 10% variation from baseline with P >0.05, we found that stability duration in plasma was 5 days for sVCAM-1 and sICAM-1 and at least 2 days for sE-selectin at 4, 21, and 30 °C and 5 days for CRP at 4 and 21 °C and 1 day at 30 °C. Stability duration in whole blood was 5 days for sVCAM-1 and sICAM-1 and at least 2 days for sE-selectin at 4, 21, and 30 °C and 5 days for CRP at 4 and 21 °C and 2 days at 30 °C. sP-selectin was not stable in plasma or whole blood. sICAM-1, sVCAM-1, CRP, and sE-selectin were stable after 5 freeze-thaw cycles.

Conclusions: sVCAM-1, sICAM-1, and CRP are stable in plasma or whole blood at 4 and 21 °C for at least 3 days and sE-selectin for 2 days. sP-selectin is not stable and therefore requires immediate assay.






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