HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: clinchem.2006.081372v1 53/3/399    most recent Submit an electronic Letter to the Editor about this paper Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Purwosunu, Y. Articles by Okai, T. Search for Related Content PubMed PubMed Citation Articles by Purwosunu, Y. Articles by Okai, T. Related Collections Other Areas of Clinical Chemistry Molecular Diagnostics and Genetics Endocrinology and Metabolism

Cell-Free mRNA Concentrations of Plasminogen Activator Inhibitor-1 and Tissue-Type Plasminogen Activator Are Increased in the Plasma of Pregnant Women with Preeclampsia

¹ Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
² Department of Obstetrics and Gynecology, University of Indonesia, Cipto Mangunkusumo National Hospital, Jakarta, Indonesia.
³ Department of Histology and Embryology Division of Prenatal Medicine, University of Bologna, Bologna, Italy.

^aAddress correspondence to this author at: Department of Obstetrics and Gynecology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, Japan. Fax 81-33784-8355; e-mail sekizawa{at}med.showa-u.ac.jp.

Background: Detection of placental mRNA in maternal plasma has been reported in high-risk pregnancies. We attempted to investigate the concentrations of plasminogen activator inhibitor-1 (PAI-1) and tissue-type plasminogen activator (tPA) mRNA in maternal plasma in preeclampsia.

Methods: Peripheral blood samples were obtained from healthy pregnant women before and after delivery and also from women with or without preeclampsia. Plasma was isolated from these samples, and RNA was extracted. Plasma PAI-1 and tPA mRNA concentrations were then measured by use of reverse transcription PCR assays. The concentrations were converted into multiples of the median (MoM) of the controls adjusted for gestational age. Data were stratified and analyzed according to the clinical severity of preeclampsia and quantitative distribution of blood pressure and proteinuria.

Results: The median (minimum–maximum) PAI-1 mRNA MoM values for women with preeclampsia and controls were 2.48 (0.82–8.53) and 1.00 (0.41–2.33), respectively, whereas the median (minimum–maximum) tPA mRNA MoM values were 3.33 (1.01–10.58) and 1.00 (0.95–1.20), respectively. The concentrations of both PAI-1 and tPA mRNA were significantly increased in cases of preeclampsia, compared with controls (P <0.0001). The MoM values of both mRNA species were directly correlated with the severity of preeclampsia and were greatest among a subgroup of hemolysis, increased liver enzymes, and low platelets pregnancies.

Conclusion: Maternal plasma PAI-1 and tPA mRNAs are significantly increased in patients with preeclampsia and are positively correlated with the severity of preeclampsia.

The following articles in journals at HighWire Press have cited this article:

				S. Okazaki, A. Sekizawa, Y. Purwosunu, A. Farina, N. Wibowo, and T. Okai Placenta-Derived, Cellular Messenger RNA Expression in the Maternal Blood of Preeclamptic Women Obstet. Gynecol., November 1, 2007; 110(5): 1130 - 1136. [Abstract] [Full Text] [PDF]

				K. Miura, K. Yamasaki, S. Miura, K.-i. Yoshiura, T. Shimada, D. Nakayama, N. Niikawa, and H. Masuzaki Circulating Cell-Free Placental mRNA in the Maternal Plasma as a Predictive Marker for Twin-Twin Transfusion Syndrome Clin. Chem., June 1, 2007; 53(6): 1167 - 1168. [Full Text] [PDF]