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Clinical Chemistry 53: 629-635, 2007. First published February 15, 2007; 10.1373/clinchem.2006.079194
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Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2007;53:629-635.)
© 2007 American Association for Clinical Chemistry, Inc.

Proteomics and Protein Markers

Protein Profiling of Microdissected Pancreas Carcinoma and Identification of HSP27 as a Potential Serum Marker

Christian Melle1, Günther Ernst1, Niko Escher1, Daniel Hartmann2, Bettina Schimmel1, Annett Bleul1, Heike Thieme1, Roland Kaufmann3, Klaus Felix2, Helmut M. Friess2, Utz Settmacher3, Merten Hommann3, Konrad K. Richter3, Wolfgang Daffner3, Horst Täubig4, Thomas Manger4, Uwe Claussen1 and Ferdinand von Eggeling1,a

1 Core Unit Chip Application, Institute of Human Genetics and Anthropology, Medical Faculty at the Friedrich Schiller University Jena, Jena, Germany.
2 Department of General Surgery, University of Heidelberg, Heidelberg, Germany.
3 Department of General, Visceral and Vascular Surgery, Medical Faculty at the Friedrich Schiller University Jena, Jena, Germany.
4 SRH Wald-Klinikum Gera, Department of General and Visceral Surgery, Gera, Germany.

aAddress correspondence to this author at: Institut für Humangenetik und Anthropologie, CUCA, 07740 Jena, Germany. Fax 0049-0-3641-935518; e-mail fegg{at}mti.uni-jena.de.

Background: Patients with pancreatic adenocarcinomas have a poor prognosis because of late clinical manifestation and the tumor’s aggressive nature. We used proteomic techniques to search for markers of pancreatic carcinoma.

Methods: We performed protein profiling of microdissected cryostat sections of 9 pancreatic adenocarcinomas and 10 healthy pancreatic tissue samples using ProteinChip technology (surface-enhanced laser desorption/ionization). We identified proteins by use of 2-dimensional gel electrophoresis, peptide fingerprint mapping, and immunodepletion and used immunohistochemistry for in situ localization of the proteins found. We used ELISA to quantify these proteins in preoperative serum samples from 35 patients with pancreatic cancer and 37 healthy individuals.

Results: From among the differentially expressed signals that were detected by ProteinChip technology, we identified 2 proteins, DJ-1 and heat shock protein 27 (HSP27). We then detected HSP27 in sera of patients by use of ELISA, indicating a sensitivity of 100% and a specificity of 84% for the recognition of pancreatic cancer.

Conclusions: The detection of DJ-1 and HSP27 in pure defined tissue and the retrieval of HSP27 in serum by antibody-based methods identifies a potential marker for pancreatic cancer.




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