Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 53: 1380-1383, 2007. First published May 10, 2007; 10.1373/clinchem.2006.084764
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow 084764.Supplemental Data
Right arrow All Versions of this Article:
clinchem.2006.084764v1
53/7/1380    most recent
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koskela, A.
Right arrow Articles by Adlercreutz, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koskela, A.
Right arrow Articles by Adlercreutz, H.
Related Collections
Right arrow Nutrition
(Clinical Chemistry. 2007;53:1380-1383.)
© 2007 American Association for Clinical Chemistry, Inc.

Technical Briefs

Quantification of Alkylresorcinol Metabolites in Urine by HPLC with Coulometric Electrode Array Detection

Anja Koskela1, Anna-Maria Linko-Parvinen1,a, Perttu Hiisivuori1,2, Adile Samaletdin1, Afaf Kamal-Eldin3, Matti J. Tikkanen1,2 and Herman Adlercreutz1

1 Institute for Preventive Medicine, Nutrition and Cancer, Folkhälsan Research Center and Division of Clinical Chemistry, University of Helsinki, Helsinki, Finland;
2 Department of Medicine, University of Helsinki, Helsinki, Finland;
3 Department of Food Science, Swedish University of Agricultural Science (SLU), Uppsala, Sweden;

aAddress correspondence to this author at: Institute for Preventive Medicine, Nutrition and Cancer, Folkhälsan Research Center and Division of Clinical Chemistry, PO Box 63, FIN-00014 University of Helsinki, Finland; fax 358-9-19125452, e-mail anna.linko{at}helsinki.fi


Abstract

Background: Whole-grain rye and wheat cereals contain high amounts of alkylresorcinols (ARs), phenolic lipids. ARs can be quantified in plasma. Two recently identified urinary AR metabolites, 3,5-dihydroxyphenylbenzoic acid (DHBA) and 3-(3,5-dihydroxyphenyl)-1-propanoic acid (DHPPA), may be useful as biomarkers of intake of whole-grain rye and wheat.

Methods: We evaluated 4 pretreatment protocols for quantifying urinary DHBA and DHPPA using HPLC coupled with a coulometric electrode array detector. Syringic acid was used as the internal calibrator.

Results: Measured urinary concentrations of DHBA and DHPPA were 0.8–115 µmol/L. The mean recoveries of all added concentrations were 85%–104% for DHBA and 86%–99% for DHPPA, depending on the degree of the purification. The protocol versions with less purification correlated well with the protocol including highest purification. The correlation coefficients (r2) were 0.9699–0.8153 for DHBA and 0.9854–0.8371 for DHPPA.

Conclusion: Although the protocol with the most purification steps was most specific, all protocols were suitable for measuring DHBA and DHPPA in urine. The rapid protocol with simple hydrolysis could be used in large-scale clinical studies. Additional investigation is needed to clarify whether these metabolites are useful biomarkers of whole-grain intake and helpful in the exploration of its association with human diseases.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the American Association for Clinical Chemistry.