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Clinical Chemistry 53: 1495-1502, 2007. First published June 28, 2007; 10.1373/clinchem.2007.086975
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(Clinical Chemistry. 2007;53:1495-1502.)
© 2007 American Association for Clinical Chemistry, Inc.

Automation and Analytical Techniques

A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

Jie Wu1, Feng Yan2, Jinhai Tang2, Chun Zhai1 and Huangxian Ju1,a

1 Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, Peoples Republic of China.
2 Jiangsu Institute of Cancer Prevention and Cure, Nanjing 210009, Peoples Republic of China.

aAddress correspondence to this author at: Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, Peoples Republic of China. Fax 86-25-83593593; e-mail hxju{at}nju.edu.cn.

Background: Automated and convenient multianalyte detection with high throughput is increasingly needed in clinical diagnosis. We developed a disposable 4-by-2 array for programmed simultaneous amperometric immunoassay of 4 tumor markers.

Methods: We used a screen-printed technique, 1-step immobilization method, and flow injection technique. We immobilized carcinoembryonic antigen, {alpha}-fetoprotein, β-human choriogonadotropin, and carcinoma antigen 125 as model analytes in a redox mediator–grafted, biopolymer-modified, screen-printed carbon electrode array to capture corresponding horseradish peroxidase-labeled antibodies in competitive immunoreactions. The simultaneous multianalyte immunoassay was automatically carried out to amperometrically monitor the mediator-catalyzed enzymatic response to hydrogen peroxide, which decreased in proportion to the concentrations of analytes in samples.

Results: The multianalyte immunosensor array had a throughput of 60 samples/h and allowed simultaneous detection of carcinoembryonic antigen, {alpha}-fetoprotein, β-human choriogonadotropin, and carcinoma antigen 125 in clinical serum samples with concentrations up to 188 µg/L, 250 µg/L, 266 IU/L, and 334 kIU/L, respectively. The detection limits (limits of the blank, mean of blank plus 3 SD) were 1.1 µg/L, 1.7 µg/L, 1.2 IU/L, and 1.7 kIU/L. The inter- and intraassay imprecision (CVs) of the immunosensor arrays were <7.8% and <9.0%, respectively. The immunosensor arrays were stable for 28 days.

Conclusions: This newly constructed immunosensor array provides a simple, automated, simultaneous multianalyte immunoassay with high throughput, short analytical time, and sufficiently low detection limits for clinical application. This method offers the capability of miniaturizing the multianalyte detection device.




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J. Wu, F. Yan, X. Zhang, Y. Yan, J. Tang, and H. Ju
Disposable Reagentless Electrochemical Immunosensor Array Based on a Biopolymer/Sol-Gel Membrane for Simultaneous Measurement of Several Tumor Markers
Clin. Chem., September 1, 2008; 54(9): 1481 - 1488.
[Abstract] [Full Text] [PDF]


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