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Clinical Chemistry 54: 1696-1704, 2008. First published August 21, 2008; 10.1373/clinchem.2007.101741
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(Clinical Chemistry. 2008;54:1696-1704.)
© 2008 American Association for Clinical Chemistry, Inc.

Cancer Diagnostics

Prognostic Value of Mature MicroRNA-21 and MicroRNA-205 Overexpression in Non–Small Cell Lung Cancer by Quantitative Real-Time RT-PCR

Athina Markou1, Emily G. Tsaroucha2, Loukas Kaklamanis3, Marianthi Fotinou2, Vassilis Georgoulias4 and Evi S. Lianidou1,a

1 Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece; 2 "Sotiria" General Hospital for Chest Diseases, Athens, Greece; 3 Department of Pathology, Onassis Cardiac Surgery Center, Athens, Greece; 4 Department of Medical Oncology and Laboratory of Tumor Cell Biology, University Hospital of Heraklion, School of Medicine, University of Crete, Greece.

aAddress correspondence to this author at: Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Greece. Fax + 30 210 7274750; e-mail lianidou{at}chem.uoa.gr.

Background: microRNA (miRNA) expression profiles are being intensively investigated for their involvement in carcinogenesis. We evaluated the prognostic value of mature microRNA-21 (miR-21) and mature microRNA-205 (miR-205) overexpression in non–small cell lung cancer (NSCLC).

Patients and methods: We studied 48 pairs of NSCLC fresh frozen tissue specimens collected at time of surgery and before chemotherapy. Highly specific amplification and quantification of mature miR-21 and mature miR-205 was achieved using looped real time RT-PCR.

Results: miRNA expression, determined by real time RT-PCR, was defined by {Delta}{Delta}Ct measurements. We detected overexpression of mature miR-21 in 25 (52.0%) of the 48 NSCLC paired specimens and overexpression of miR-205 in 31 (64.6%). Overexpression was assessed after comparison of miRNA expression in NSCLC tissues and in their corresponding noncancerous tissues with respect to U6 expression. During the follow-up period, 29 of 48 (60.4%) patients relapsed, and 23 of 48 died (47.9%). Mature miR-21 was upregulated in 16 of 29 (55.2%) patients who relapsed and 15 of 23 (65.2%) patients who died. Mature miR-205 was overexpressed in 19 of 29 patients who relapsed (65.5%) and 15 of 23 patients who died (65.2%). Mature miR-21 overexpression correlated with overall survival (OS) of the patients (P = 0.027), whereas overexpression of mature miR-205 did not.

Conclusions: Our results suggest that overexpression of mature miR-21 is an independent negative prognostic factor for OS in NSCLC patients.




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