Clinical Chemistry
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Clinical Chemistry 54: 2063-2066, 2008. First published October 2, 2008; 10.1373/clinchem.2008.112219
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(Clinical Chemistry. 2008;54:2063-2066.)
© 2008 American Association for Clinical Chemistry, Inc.

Brief Communications

Seminal Oligouridinosis: Low Uridine Secretion as a Biomarker for Infertility in Spinal Neurotrauma

Anthony D. Maher1,2, Prasad Patki2,2, John C. Lindon1, Elizabeth J. Want1, Elaine Holmes1, Michael Craggs2,3,a and Jeremy K. Nicholson1,a

1 Department of Biomolecular Medicine, SORA Division, Faculty of Medicine, Imperial College London, London, UK; 2 Department of Neuro-urology, Royal National Orthopaedic Hospital, Stanmore, Middlesex, UK; 3 Division of Surgery and Interventional Sciences, University College London and Spinal Research Centre, Royal National Orthopaedic Hospital, Stanmore, Middlesex, UK.

aaddress correspondence to: M.C. at Department of Neuro-urology, Royal National Orthopaedic Hospital, Stanmore, Middlesex, HA7 4LP, UK. Fax +44 20 8909 5343; e-mail michael.craggs{at}ucl.ac.uk. J.K.N. at Department of Biomolecular Medicine, SORA Division, Faculty of Medicine, Imperial College London, SW7 2AZ, UK. E-mail j.nicholson{at}imperial.ac.uk. This report was presented at the American Urological Association, 2007 Annual Meeting, Anaheim, California.


Abstract

Background: Compromised sexual health is a major rehabilitative barrier for men with lower–spinal cord injury (SCI). Although studies have revealed decreased sperm motility, the quantitative biochemical changes that underlie the infertility mechanism remain poorly understood.

Methods: We employed a nontargeted approach combining 800 MHz hydrogen nuclear magnetic resonance (1H NMR) spectroscopy and ultra-performance liquid chromatography–mass spectrometry (UPLC-MS) with pattern recognition methods to analyze seminal fluid metabolite profiles in 10 men with and 8 without SCI above thoracic vertebra 10 (T10).

Results: The metabolic phenotype for SCI could be predicted from the 1H NMR data. The median concentration of uridine in fertile controls was 1.55 mmol/L (range 1.0–5.0 mmol/L), but was undetectable by both NMR and MS in all but 2 individuals from the SCI group, one who later fathered a child without assisted fertility techniques.

Conclusions: We hypothesize that uridine is likely to be an essential precursor to metabolites required for capacitation and is a potential marker for the prognosis of post-SCI functional fertility recovery. We derived the term "seminal oligouridinosis" to describe this newly identified condition.






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