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Clinical Chemistry 54: 907-910, 2008; 10.1373/clinchem.2007.100941
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(Clinical Chemistry. 2008;54:907-910.)
© 2008 American Association for Clinical Chemistry, Inc.

Brief Communications

Estimation of LDL-Associated Apolipoprotein B from Measurements of Triglycerides and Total Apolipoprotein B

Arthur M. Bacaa and G. Russell Warnick

1 Berkeley Heartlab, Inc., Alameda, CA;

aaddress correspondence to this author at: Berkeley Heartlab, Inc., 960 Atlantic Ave., Suite 100, Alameda, CA 94501. E-mail abaca{at}bhlinc.com.


Abstract

Background: VLDL and chylomicrons may interfere with measurements of apolipoprotein B (apo B) on LDL particles. Ultracentrifugation of samples enriched in chylomicrons and VLDL and subsequent measurement of apo B in the infranate fraction [density (d) = 1.006] removes this interference. This apo B fraction is called "LDL–apo B."

Methods: We retrospectively analyzed 64 895 measurements of triglycerides, total apo B, and LDL–apo B. Samples were ultracentrifuged, and 3 commercially available immunoassays that use different antibodies were used to measure LDL–apo B in the 1.006 infranate fraction.

Results: After adjusting for triglyceride concentration, we found total apo B and LDL–apo B measurements to be strongly correlated. We derived a simple linear equation for calculating LDL–apo B concentration (in milligrams per deciliter) from measurements of total apo B and triglycerides: LDL–apo B = apo B – 10 mg/dL – triglycerides/32. This equation accurately predicts LDL–apo B values within ±12% of the measured value in 75% of cases.

Conclusions: Our equation provides a convenient means of estimating LDL–apo B from commonly available measurements of total apo B and triglycerides without the need for ultracentrifugation. LDL–apo B measurements were also independent of the different apo B antibodies in the 3 assays used in this study. An equation that predicts LDL–apo B particle number may be useful, regardless of the apo B assay used.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
J. R. Burnett and G. F. Watts
Estimating LDL ApoB: Infomania or Clinical Advance?
Clin. Chem., May 1, 2008; 54(5): 782 - 784.
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