Clinical Chemistry
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Clinical Chemistry 54: 1080-1084, 2008; 10.1373/clinchem.2007.102319
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(Clinical Chemistry. 2008;54:1080-1084.)
© 2008 American Association for Clinical Chemistry, Inc.

Brief Communications

Rapid Determination of Monozygous Twinning with a Microfabricated Capillary Array Electrophoresis Genetic-Analysis Device1

Stephanie H. I. Yeung1,2, Igor L. Medintz2,2, Susan A. Greenspoon3 and Richard A. Mathies1,4,a

1 UCSF/UCB Joint Graduate Group in Bioengineering, University of California, Berkeley, CA; 2 Center for Bio/Molecular Science and Engineering, Code 6900, US Naval Research Laboratory, Washington, DC; 3 Virginia Department of Forensic Science, DNA Unit, Richmond, VA;4 Department of Chemistry, University of California, Berkeley, CA

aaddress correspondence to this author at: Department of Chemistry, University of California, Berkeley, CA 94720. e-mail Rich{at}zinc.cchem.berkeley.edu


Abstract

Background: Microfabricated genetic-analysis devices have great potential for delivering complex clinical diagnostic technology to the point of care. As a demonstration of the potential of these devices, we used a microfabricated capillary array electrophoresis (µCAE) instrument to rapidly characterize the familial and genotypic relationship of twins who had been assigned fraternal (dizygous) status at birth.

Methods: We extracted the genomic DNA from buccal samples collected from the twin sons, the parents, another sibling, and an unrelated control individual. We then carried out multiplex PCR amplification of sequences at 16 short tandem repeat loci commonly used in forensic identity testing. We simultaneously separated the amplicons from all of the individuals on a µCAE device and fluorescently detected the amplicons with single-base resolution in <30 min.

Results: The genotypic analysis confirmed the identical status of the twins and revealed, in conjunction with the medical data, that their twin status arose from the rarer dichorionic, diamniotic process.

Conclusions: The ability to rapidly analyze complex genetic samples with µCAE devices demonstrates that this approach can help meet the growing need for rapid genetics-based diagnostics.






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Copyright © 2008 by the American Association for Clinical Chemistry.