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Special Report |
1 Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK; 2 Genomics Core Facility, EMBL Heidelberg, Heidelberg, Germany; 3 Centre for Virology, Department of Infection, University College London, London, UK; 4 Department of Virology, UCL Hospitals NHS Foundation Trust, London, UK; 5 Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium; 6 Centre for Infectious Diseases, University College London, London, UK; 7 TATAA Biocenter, Göteborg, Sweden; 8 Institute of Biotechnology AS CR, Prague, Czech Republic; 9 Sequenom, San Diego, California, USA; 10 Sigma–Aldrich, Haverhill, UK; 11 Physiology Weihenstephan, Technical University Munich, Freising, Germany; 12 Quantitative Genomics Core Laboratory, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, Texas, USA; 13 Department of Pathology, University of Utah, Salt Lake City, Utah, USA; 14 ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.
aAddress correspondence to this author at: 3rd Floor Alexandra Wing, The Royal London Hospital, London E1 1BB, UK. Fax +44-(0)20-7377 7283; e-mail s.a.bustin{at}qmul.ac.uk.
Background: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader’s ability to evaluate critically the quality of the results presented or to repeat the experiments.
Content: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement.
Summary: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.
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N. Hosono, M. Kato, K. Kiyotani, T. Mushiroda, S. Takata, H. Sato, H. Amitani, Y. Tsuchiya, K. Yamazaki, T. Tsunoda, et al. CYP2D6 Genotyping for Functional-Gene Dosage Analysis by Allele Copy Number Detection Clin. Chem., August 1, 2009; 55(8): 1546 - 1554. [Abstract] [Full Text] [PDF]
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