Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 55: 964-971, 2009. First published March 5, 2009; 10.1373/clinchem.2008.116582
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
clinchem.2008.116582v1
55/5/964    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, Y.
Right arrow Articles by Yu, R.-Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, Y.
Right arrow Articles by Yu, R.-Q.
(Clinical Chemistry. 2009;55:964-971.)
© 2009 American Association for Clinical Chemistry, Inc.

Automation and Analytical Techniques

Prostate Specific Antigen Detection Using Microgapped Electrode Array Immunosensor with Enzymatic Silver Deposition

Yong Huang1, Tai-Hong Wang1, Jian-Hui Jiang1,a, Guo-Li Shen1 and Ru-Qin Yu1

1 State Key Laboratory for Chemo/biosensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.

aAddress correspondence to this author at: State Key Laboratory for Chemo/biosensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. Fax +86 731 8821916; e-mail jianhuijiang{at}hnu.cn.

Background: Analysis of trace proteins plays an essential role in the fields of biomedical research and clinical diagnosis. Development of methods for the detection of proteins at very low concentrations has historically been a challenge in immunochemistry. We have developed an electrical immunosensor for the detection of prostate specific antigen (PSA).

Methods: The electrical immunosensor uses a microgapped interdigitated electrode array (MGIDEA) based on enzymatic silver deposition reaction. The deposition of silver was dispersed over the microgaps and allows the microgapped interdigitated electrodes to be electrically connected, resulting in an increase in electrical conductance of MGIDEA that is used to quantify the analyte concentration. We used this electrical immunosensor to measure PSA in human serum samples from patients with prostate diseases.

Results: This electrical immunosensor exhibited a linear response with PSA concentrations over a 6-decade range from 1.0 pg/L to 1.0 µg/L, with detection limit of 0.9 pg/L. PSA concentrations using this immunosensor agreed within 10% of those obtained using a commercial chemiluminescent immunoassay.

Conclusions: The MGIDEA method has characteristics (analyte specific, low background, low limit of detection) that provide potential for molecular detection in various biomedical areas.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the American Association for Clinical Chemistry.