Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 55: 978-985, 2009. First published March 5, 2009; 10.1373/clinchem.2008.118299
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
clinchem.2008.118299v1
55/5/978    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dähnrich, C.
Right arrow Articles by Komorowski, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dähnrich, C.
Right arrow Articles by Komorowski, L.
(Clinical Chemistry. 2009;55:978-985.)
© 2009 American Association for Clinical Chemistry, Inc.

Clinical Immunology

New ELISA for Detecting Primary Biliary Cirrhosis–Specific Antimitochondrial Antibodies

Cornelia Dähnrich1, Albert Pares2, Llorenç Caballeria2, Anke Rosemann1, Wolfgang Schlumberger1, Christian Probst3, Maria Mytilinaiou4, Dimitrios Bogdanos4,1, Diego Vergani4, Winfried Stöcker3 and Lars Komorowski3,a,1

1 EUROIMMUN AG, Lübeck, Germany; 2 Liver Unit, Institut de Malalties Digestives CIBEREHD, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain; 3 Department of Immunobiochemical Research, Institute for Experimental Immunology affiliated to EUROIMMUN AG, Lübeck, Germany;4 Institute of Liver Studies, King’s College London School of Medicine at King’s College Hospital, London, U.K.

aAddress correspondence to this author at: Department of Immunobiochemical Research, Institute for Experimental Immunology affiliated to EUROIMMUN AG, Seekamp 31, 23560 Lübeck, Germany. Fax +49 451 5855391; e-mail l.komorowski{at}euroimmun.de.

Background: Antimitochondrial antibodies specific for primary biliary cirrhosis (PBC) target the E2 subunits of 2-oxo acid dehydrogenase complexes, in particular the pyruvate dehydrogenase complex (PDC)-E2. Their antigen-specific detection relies on conventional ELISA using purified PDC. More recent assays have employed a hybrid containing the 3 E2-subunits (MIT3). Some PBC sera react with one or the other preparation, suggesting the presence of nonoverlapping epitopes.

Methods: We have developed an ELISA (anti-M2-3E) using a mixture of purified PDC and MIT3 as antigenic targets. We compared this assay to anti-MIT3 alone, conventional anti-PDC, and indirect immunofluorescence using 173 PBC and 247 disease controls.

Results: The anti-M2-3E ELISA showed a 93.6% diagnostic sensitivity compared with 91.3%, 83.8%, and 87.3% for MIT3, purified PDC, or indirect immunofluorescence, respectively, when all specificities are set to 98.8%. By immunoblotting, anti-M2-3E–positive sera unreactive to purified PDC recognized recombinant E2-subunits of the other 2 complexes, whereas those with no reactivity to MIT3 immunofixed PDC subunits E1{alpha} or E1β.

Conclusions: The diagnostic accuracy of the anti-M2-3E ELISA for detection of antibodies to 2-oxo acid dehydrogenase complexes exceeds that of conventional ELISA and IFL; its novelty derives from the combination of the MIT3 hybrid and purified PDC.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the American Association for Clinical Chemistry.