Clinical Chemistry
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Clinical Chemistry 55: 1061-1066, 2009. First published March 26, 2009; 10.1373/clinchem.2007.100008
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(Clinical Chemistry. 2009;55:1061-1066.)
© 2009 American Association for Clinical Chemistry, Inc.

Mini-Review

Steroid Hormone Analysis by Tandem Mass Spectrometry

Steven J. Soldin1,2,a and Offie P. Soldin1,3,4

1 Department of Medicine; and 2 Department of Pharmacology and General Clinical Research Center; 3 Lombardi Department of Oncology, Comprehensive Cancer Center; and 4 Department of Physiology and Biophysics, Georgetown University, Washington, DC.

aAddress correspondence to this author at: Bioanalytical Core Laboratory, Room GM12A, Preclinical Science Building, Georgetown University, 3900 Reservoir Road NW, Washington, DC 20007. E-mail sjs44{at}georgetown.edu


Abstract

Background: New high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) methods are among the most successful approaches to improve specificity problems inherent in many immunoassays.

Content: We emphasize problems with immunoassays for the measurement of steroids and review the emerging role of LC-MS/MS in the measurement of clinically relevant steroids. The latest generation of tandem mass spectrometers has superior limits of quantification, permitting omission of previously employed derivatization steps. The measurement of steroid profiles in the diagnosis and treatment of congenital adrenal hyperplasia, adrenal insufficiency, chronic pelvic pain and prostatitis, oncology (breast cancer), and athletes has important new applications.

Conclusions: LC-MS/MS now affords the specificity, imprecision, and limits of quantification necessary for the reliable measurement of steroids in human fluids, enhancing diagnostic capabilities, particularly when steroid profiles are available.






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Copyright © 2009 by the American Association for Clinical Chemistry.