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Clinical Chemistry 6: 16-22, 1960;
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Clinical Chemistry, Vol 6, 16-22, Copyright © 1960 by the American Association for Clinical Chemistry

A Rapid Method for the Estimation of Urinary 17-Ketosteroids

F. Rappaport 1, J. Fischl 2, and N. Pinto 2

1 Deceased July 17, 1959.
2 Department of Chemistry, Beilinson Hospital, Petach Tikva and "Assaf Harofe" Government Hospital, Zrifin, Israel.

A rapid method for the estimation of urinary 17-ketosteroids, based on the Zimmerman reaction, is presented. The color complex due to ketosteroids is selectively extracted with chloroform. Turbidity of chloroform extract is avoided by the addition of polyethyleneglycol.

Submitted on June 4, 1959




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