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Clinical Chemistry 6: 148-152, 1960;
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Clinical Chemistry, Vol 6, 148-152, Copyright © 1960 by the American Association for Clinical Chemistry

Notes on Methemoglobin Determination

Thomas Leahy 1 and Roger Smith 1

1 Chemistry Department of the USAREUR Medical Laboratory, APO 180, New York, N. Y.

The Evelyn-Malloy procedure for the determination of methemoglobin in blood has been modified for use with a Coleman Jr. spectrophotometer by using a lower dilution of blood to reduce the effective instrument error from a possible 10 per cent to a possible 3 per cent.

The method has been further modified to give results directly as the per cent methemoglobin of the total blood pigment.

The normal range for this determination appears to be up to 2 per cent of the total pigment present as methemoglobin.

A statistically significant difference was observed between the means of the methemoglobin percentages of a normal group and a group of persons whose occupation placed them in frequent contact with petroleum fuels.




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Copyright © 1960 by the American Association for Clinical Chemistry.