Clinical Chemistry
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Clinical Chemistry 6: 208-215, 1960;
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Clinical Chemistry, Vol 6, 208-215, Copyright © 1960 by the American Association for Clinical Chemistry

Colorimetric Determination of Serum Isocitric Dehydrogenase

Comparison with a Spectrophotometric Procedure

Thomas H. Taylor 1 and Michael E. Friedman 1

1 Dr. Milton J. Goodfriend Research Laboratory, Department of Laboratories, Lebanon Hospital, New York, and the Pack Medical Foundation, New York.

A colorimetric assay of serum isocitric dehydrogenase has been described in which the alpha-ketoglutaric acid formed is determined as the hydrazone. A standard unit of lCD activity is defined as millimicromoles KG-A formed per milliliter serum per hour of incubation at 37°.

Analyses of KGA and TPNH as reaction products show that 1.58 moles of KGA are formed for each mole of TPNH under the conditions of this experiment.

Submitted on November 7, 1959




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Copyright © 1960 by the American Association for Clinical Chemistry.