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Clinical Chemistry 6: 216-222, 1960;
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Clinical Chemistry, Vol 6, 216-222, Copyright © 1960 by the American Association for Clinical Chemistry

Contamination of Malic Dehydrogenase with Apotransaminase

Hyman J. Zimmerman 1, Ivan J. Silverberg 1, and Michael West 1

1 Departments of Medicine, The Chicago Medical School, The Mount Sinai Hospital, and The West Side Veterans Administration Hospital, Chicago, Ill.

Studies of 4 commercially available malic dehydrogenase preparations have shown very small amounts of transaminase activity. Incubation of these preparations with pyridoxal 5-phosphate has revealed an enhanced transaminase activity. The data reported here suggest that the enhanced transaminase activity represents apotransaminase contaminating the malic dehydrogenase preparation.

Submitted on July 13, 1959






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