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Clinical Chemistry 6: 243-253, 1960;
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Clinical Chemistry, Vol 6, 243-253, Copyright © 1960 by the American Association for Clinical Chemistry

Determination of Cholesterol with Special Reference to the Use of p-Toluenesulfonic Acid

L. A. Wright 1, D. B. Tonks 1, and R. H. Allen 1

1 Clinical Laboratories, Laboratory of Hygiene, Department of National Health and Welfare, Ottawa, Ontario, Canada.

1. The Pearson, Stern, and McGavack method for the determination of cholesterol was found to be rapid and suitable for routine clinical purposes. It gave, within experimental error, total cholesterol values in agreement with the method of Bloor.

2. A proposed modification of the method, providing for a final volume of 6.3 ml., and a shorter operational time, was found to give values in excellent agreement with the original method.

3. Sulfosalicylic acid may be used as a substitute for p-toluenesulfonic acid. However, no evidence of the excessive reactivity reported from one source has been experienced at any time in this laboratory with the p-toluenesulfonic acid reagent.

4. Similar optical densities were obtained from equimolar quantities of cholesterol and cholesteryl palmitate when the Pearson, Stern, and McGavack method was used.

5. Serum cholesterol values for 61 normal males were found to range from 150 to 320 mg./100 ml. of serum.

Submitted on August 11, 1959






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Copyright © 1960 by the American Association for Clinical Chemistry.