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Clinical Chemistry 6: 327-331, 1960;
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Clinical Chemistry, Vol 6, 327-331, Copyright © 1960 by the American Association for Clinical Chemistry

Toxicologic Studies on Hydrocarbons. VI. A Colorimetric Method for the Determination of Kerosine in Blood

Horace W. Gerarde 1 and Paul Skiba 1

1 Medical Research Division, Esso Research and Engineering Co., Linden, N. J., and The Bureau of Biological Research, Rutgers State University, New Brunswick, N. J.

A photoelectric colorimetric method is described for the quantitative determination of kerosine in blood. The procedure involves hemolysis of 5 ml. of the sample followed by extraction of the kerosine with carbon tetrachloride. The extract is reacted with a formaldehyde- sulfuric acid reagent to produce a characteristic color. The intensity of this color is measured photometrically, and the concentration of kerosine is determined by reference to a previously prepared calibration curve. Concentrations as low as 10 ppm can be conveniently determined.

Submitted on October 16, 1959






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Copyright © 1960 by the American Association for Clinical Chemistry.