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Clinical Chemistry 6: 429-433, 1960;
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Clinical Chemistry, Vol 6, 429-433, Copyright © 1960 by the American Association for Clinical Chemistry

Assay of Fibrinogen in Small Blood Samples

H. D. Wycoff 1

1 Department of Biochemistry, Medical College of Georgia, Augusta, Ga.

A procedure is presented for the assay of fibrinogen in small samples of blood. A 0.2-ml. sample of whole blood is mixed in the syringe with 0.3 ml. of isotonic citrate-phosphate buffer and centrifuged in special tubes in which the cell volume is measured. From these measurements the dilution of the supernatant plasma is calculated. The plasma is assayed for fibrinogen and this result is corrected for dilution. Plasma fibrinogen values obtained in this way are comparable to those obtained with other methods that require larger samples of whole blood.

Submitted on November 13, 1959






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Copyright © 1960 by the American Association for Clinical Chemistry.