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Clinical Chemistry 6: 501-513, 1960;
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Clinical Chemistry, Vol 6, 501-513, Copyright © 1960 by the American Association for Clinical Chemistry

Metabolic Characterization of Polymorphonuclear Leukocytes

Frances L. Estes 1, Nancy S. Austin 1, and Joseph H. Gast 1

1 Department of Biochemistry, Baylor University College of Medicine, Houston, Texas.

The viability and cellular integrity of polymorphonuclear leukocytes obtained from intraperitoneal exudates are maintained in the absence of serum or added organic material. Under these conditions the endogenous oxygen consumption has a p11 optimum of 7.0 to 7.2. In the presence of glucose the oxygen consumption is inhibited and this inhibition correlates with the concentration of glucose added over a range of 0.2 to 1.0 µM. The inhibition can be overcome in part by prolonged incubation, by the addition of cytochrome C, or by succinate addition.






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