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Clinical Chemistry 6: 564-571, 1960;
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Clinical Chemistry, Vol 6, 564-571, Copyright © 1960 by the American Association for Clinical Chemistry

Determination of Intracellular Magnesium by an Automatic Titration Procedure

Donald A. Sones 1, Warren F. McGuckin 1, and Khalil G. Wakim 1

1 Mayo Clinic and Mayo Foundation, Rochester, Minn.

Our experiences with the automatic titration method of quantitating serum calcium and magnesium are presented. In addition, modifications of the original procedure to allow determination of intraerythrocyte concentrations of magnesium are described.

It appears that adoption of a separate magnesium standard in addition to the calcium standard, plus certain other minor modifications designed to minimize the variables inherent in the procedure, provides a significant increase in accuracy and reproducibility of results.

Values for intraerythrocyte magnesium concentration in 9 normal dogs were found to range from 4.69 to 5.77 mg./100 ml. Values for 4 dogs that received long-term administration of chlorothiazide also fell within this normal range.

Submitted on May 16, 1960






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Copyright © 1960 by the American Association for Clinical Chemistry.