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Clinical Chemistry 7: 37-53, 1961;
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Clinical Chemistry, Vol 7, 37-53, Copyright © 1961 by the American Association for Clinical Chemistry

Simplified Determination of the Lipid Components of Blood Serum

Harold V. Connerty 1, Anglis R. Briggs 1, and Edward H. Eaton Jr. 1

1 Department of Pathology, Veterans Administration Hospital, wilmington, Del.

A simplified scheme of analysis is presented for the determination of free and esterified cholesterol, the phospholipids, and the neutral fats (triglycerides) of blood serum. The hydroxamic acid reaction is used to determine total esterified fatty acids. The phospholipids are determined after acid digestion of a trichloroacetic acid precipitate of protein-phospholipid, and both total and free cholesterol are determined by a modified ferric ammonium sulfate reagent. Complete details of methods and calculations are described and discussed.

Submitted on March 18, 1960




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