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Clinical Chemistry 7: 136-142, 1961;
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Clinical Chemistry, Vol 7, 136-142, Copyright © 1961 by the American Association for Clinical Chemistry

Serum beta-Glucuronidase Assay by the Phenolphthalein Mono-beta-Glucuronide Method

Gilbert Goldstein 1

1 Department of Chemistry, Mount Sinai Hospital, New York, N. Y.

In the phenolphthalein mono-beta-glucuronide method for beta-glucuronidase assay, diminished enzyme levels have been observed when trichloroacetic acid or heat coagulation are used for deproteinization, but not when acetone is used for this purpose. Data are presented to show the effect of these reagents when blood serum is used as a source of enzyme.

The optimum pH of serum beta-glucuronidase was found to be 4.1-4.2 in acetate buffer at 37°, and the assay is performed most conveniently without protein removal.

Submitted on June 29, 1960




The following articles in journals at HighWire Press have cited this article:


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K Soltaninejad, S Shadnia, M Afkhami-Taghipour, R Saljooghi, A Mohammadirad, and M Abdollahi
Blood {beta}-glucuronidase as a suitable biomarker at acute exposure of severe organophosphorus poisoning in human
Human and Experimental Toxicology, December 1, 2007; 26(12): 963 - 966.
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Copyright © 1961 by the American Association for Clinical Chemistry.