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Clinical Chemistry 7: 265-274, 1961;
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Clinical Chemistry, Vol 7, 265-274, Copyright © 1961 by the American Association for Clinical Chemistry

Measurement of Serum Lactic Dehydrogenase

H. Alan Ells 1

1 Cardiovascular Section, Oklahoma Medical Research Institute, Oklahoma City 4, Okla.

A colorimetric method is described for the measurement of serum lactic dehydrogenase (LDH) activity. The method employs phenazine methosulfate to mediate electron transfer between reduced diphosphopyridine nucleotide and 2,6-dichloroindophenol, and allows continuous measurement of the reaction rate in the direction lactate to pyruvate. The LDH of a number of normal and pathologic sera was measured by the present method and by the ultraviolet spectrophotometric method. Agreement between the two methods is good.

Submitted on July 1, 1960






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Copyright © 1961 by the American Association for Clinical Chemistry.