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Clinical Chemistry 7: 542-545, 1961;
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Clinical Chemistry, Vol 7, 542-545, Copyright © 1961 by the American Association for Clinical Chemistry

Determination of Glucose by an Improved Enzymatic Procedure

Mary E. Washko 1 and Eugene W. Rice 1

1 Clinical Laboratory, Presbyterian and Woman's Hospitals, and the Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.

The glucose oxidase-peroxidase system for the enzymatic determination of glucose was investigated with respect to the instability of the enzyme reagent, the buffering capacity of the reaction milieu, and the stability and sensitivity of the final chromogen. An improved procedure was developed in which the enzyme solution is both stabilized and more adequately buffered by reconstitution in 40% aqueous glycerol containing 0.04 M phosphate buffer, pH 7.0. The sensitivity and stability of the colored product is markedly increased when a final sulfuric acid concentration of 4.9 N is used.

Submitted on August 12, 1960




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Copyright © 1961 by the American Association for Clinical Chemistry.