Clinical Chemistry
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Clinical Chemistry 8: 67-71, 1962;
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Clinical Chemistry, Vol 8, 67-71, Copyright © 1962 by the American Association for Clinical Chemistry

Absorption Measurements for Oxygenated and Reduced Hemoglobin in the Range 0.6—1.88 Microns

Robert B. Barlow Jr. 1 and Michael L. Polanyi 1

1 American Optical Company, Research Center, Southbridge, Mass.

Absorbance measurements are made for oxygenated and reduced hemoglobin in the range 0.6—1.88 µ for application to new methods of dye-dilution studies. Experimental results show that an isosbestic region exists between 1.3 and 1.88 µ, with the relative extinction coefficients of O2Hb and Hb equaling zero at 1.3, 1.67, and 1.77 µ.

Submitted on January 23, 1961






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Copyright © 1962 by the American Association for Clinical Chemistry.